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#celldyn2023: Andor 'SRRF-Stream'

Posted by FocalPlane, on 7 June 2023

Our recent Journal of Cell Science ‘Imaging Cell Dynamics’ meeting took place at Pestana Palace Hotel in Lisbon. In this post, we share the talk from Bruno Combettes, Microscopy Specialist at Andor, on their real time SRRF (SRRF-Stream) technology.

#celldyn2023: the benefits of using VT-iSIM

Posted by FocalPlane, on 30 May 2023

Our recent Journal of Cell Science ‘Imaging Cell Dynamics’ meeting took place at Pestana Palace Hotel in Lisbon. The meeting featured excellent talks describing a range of different microscopy techniques and their application to a plethora of questions in cell biology. You can check out our preList from the meeting on preLights. The meeting also

Leveraging biophysics to push the limits of investigating RNAs in cells: Riboglow-FLIM, a breakthrough tool for visualizing RNAs in live mammalian cells

Posted by nadia sarfraz, on 1 May 2023

Nadia Sarfraz1, Esther Braselmann1* 1 Department of Chemistry, Georgetown University, Washington, District of Columbia, USA *corresponding author braselmannlab.com Ribonucleic acids (RNAs) play a critical role in cellular function. Visualizing RNAs in space and time in the context of living cells is critical to advance our understanding of biological processes in health and disease.1 Recently, our

Bypassing 164 years of tradition with 'any immersion microscopy'

Posted by Alfred Millett-Sikking, on 24 April 2023

The optical microscope is a classic scientific instrument with a straightforward purpose: to observe objects in more detail than is possible with the naked eye. Many microscope variations exist, from the rudimentary examples of the 17th century, to modern computer controlled systems with sophisticated designs. Despite the variety, most optical microscopes reuse the same physical

Fast4DReg - to the rescue of your drifty microscopy data

Posted by Joanna Pylvänäinen, on 10 March 2023

Fast4DReg to the rescue of your 4D microscopy data In life sciences, researchers use microscopes to study living organisms, such as cells or small animals. These live cell imaging experiments are usually performed over several hours, exposing the experiment to changes in the sample and in the microscope surroundings – causing the data to drift.

VollSeg and its extension Napari plugins

Posted by varun kapoor, on 15 January 2023

In this article we present the Napari plugin VollSeg and its ongoing extension plugins that we are creating as a part of the CZI grant awarded successively to the non-profit company KapoorLabs, Paris. VollSeg is a Napari plugin for performing semantic, instance segmentation and denoising using the seed pooling approach that requires StarDist and either

TrackMate-Oneat: Auto Track correction using deep learning networks

Posted by varun kapoor, on 4 July 2022

During the tracking of motile cells, solving the problem of linking objects between two consecutive timepoints becomes even more complicated, if the cells divide or undergo cell death. In the terms of trajectories, this means the addition of trajectory branches and terminations. However, dividing and dying cells are characteristic in their shape, and leveraging this

Using an autocollimator to align 4f systems

Posted by Brian Patton, on 29 June 2022

Graeme Johnstone, Brian Patton, Department of Physics and SUPA, University of Strathclyde, Glasgow G4 0NG, United Kingdom Modern microscope systems comprise many optical elements with precise alignment tolerances. As such, as the complexity of the system increases, the challenge to align them rapidly becomes a major hurdle to implementing home-built designs. One recurring problem is

#GliaMorph - a tool to analyse glial cell morphology in 3D

Posted by Elisabeth Kugler, on 17 May 2022

Contents What is #GliaMorph? What can you do and analyse with #GliaMorph? Links to manuscripts and data Contributions and Feedback Thanks to all contributors and people providing feedback References What is GliaMorph? GliaMorph is a toolkit implemented as Fiji Macros to allow modular application to process and analyse Müller glia morphology in the retina. During

Using the Dragonfly Spinning Disk Confocal to Understand the Molecular Basis of Epigenetics

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Microscopy-related articles from our journals

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples Development 2024 151: dev202307
filoVision – using deep learning and tip markers to automate filopodia analysis J Cell Sci 2024 137: jcs261274
C omputational tools for quantifying actin filament numbers, lengths, and bundling Biology Open 2024 13: bio.060267

Microscopy-related preprint highlights

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