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Workshop report: 18th LFD Workshop in Advanced Fluorescence Imaging and Dynamics

Posted by FocalPlane, on 15 May 2025

The Laboratory for Fluorescence Dynamics ran their annual Advanced Fluorescence Dynamics Workshop in October 2024. Three of the attendees, Teemly Contreras, Irene Carlon-Andres and Razeen Shaikh were supported by JCS-FocalPlane Training Grants. They shared their experiences at the workshop here: Teemly Contreras The Advanced Fluorescence Dynamics Workshop organized by Laboratory for Fluorescence Dynamics (LFD) at

FLIMposium - Wednesday 7th May - King's College London

Posted by siân culley, on 15 April 2025

The FLIMposium is a one-day symposium dedicated to the latest advances in Fluorescence Lifetime Imaging Microscopy (FLIM). The event will take place on Wednesday, 7th of May, at the Science Gallery, Guy’s Campus, King’s College London. The symposium will feature keynote speakers and invited talks across three main themes: There will also be lightning talks and interactive breakout discussions

Microscopy preprints - applications in biology

Posted by FocalPlane, on 21 February 2025

Here is a curated selection of preprints published recently. In this post, we share preprints that use microscopy tools to answer questions in biology.

Leveraging biophysics to push the limits of investigating RNAs in cells: Riboglow-FLIM, a breakthrough tool for visualizing RNAs in live mammalian cells

Posted by nadia sarfraz, on 1 May 2023

Nadia Sarfraz1, Esther Braselmann1* 1 Department of Chemistry, Georgetown University, Washington, District of Columbia, USA *corresponding author braselmannlab.com Ribonucleic acids (RNAs) play a critical role in cellular function. Visualizing RNAs in space and time in the context of living cells is critical to advance our understanding of biological processes in health and disease.1 Recently, our

Quality assurance of segmentation results

Posted by Mara Lampert, on 13 April 2023

This blog post revolves around determining and improving the quality of segmentation results. A common problem is that this step is often omitted and done rather by the appearance of the image segmentation than by actually quantifying it. This blogpost aims to show different ways to achieve this quantification as this leads to reproducibility. Therefore,

Technology highlights - Fluorescence Lifetime Imaging (FLIM)

Posted by Johanna Bischof, on 25 November 2020

Interview with Prof. Dolf Weijers and Prof. Joris Sprakel from Wageningen University, The Netherlands Tell us a little bit about who you are and about the people involved in the work we are talking about today. This work is a collaboration between the groups of Prof. Dolf Weijers and Prof. Joris Sprakel at Wageningen University,

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Microscopy-related articles from our journals

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples Development 2024 151: dev202307
filoVision – using deep learning and tip markers to automate filopodia analysis J Cell Sci 2024 137: jcs261274
C omputational tools for quantifying actin filament numbers, lengths, and bundling Biology Open 2024 13: bio.060267

Microscopy-related preprint highlights

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