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Technology highlights - Coherent Anti-Stokes Raman Spectroscopy (CARS)

Posted by Johanna Bischof, on 28 October 2020

Interview with Antti Isomäki, PhD from the Biomedicum Imaging Unit of the University of Helsinki, Finland and Dalibor Pánek, PhD from the BIOCEV in Prague, Czech Republic. Tell us a bit about who you are and where your facility is based. AI: My name is Antti Isomäki and I am an optical physicist by training.

LSFM series – Surfing on the data freak wave! Part II: Before imaging: Know your sample (geometry)

Posted by Elisabeth Kugler, on 10 October 2020

This post, part of the blog series "LSFM series – Surfing on the data freak wave!", discusses (a) Sample preparation, (b) Light interaction with matter, (c) sample alignment and (d) checking fluorescence and calibration

Technology highlights – Structured Illumination Microscopy (SIM)

Posted by Johanna Bischof, on 30 September 2020

Interview with Ivan Novotný, Ph.D., from the Light Microscopy Core facility at the Institute of Molecular Genetics, in Prague, Czech Republic. Please tell us a bit about yourself and the facility where you work. I am employed as an imaging specialist at the Light Microscopy Core facility at the Institute of Molecular Genetics of the

Light microscopy core facility support specialist, Biomedicum Imaging Unit, University of Helsinki, Finland

Posted by Kirstin Vonderstein, on 15 September 2020

We are looking for a highly motivated Laboratory Engineer / light microscopy core facility support specialist for the Biomedicum Imaging Unit at the University of Helsinki, Faculty of Medicine. You would be joining a team of four support personnel and strengthening our capability to offer light microscopy and image analysis support for a community of

Bioimage Analysis in FIJI - Resource List

Posted by Wilson Adams, on 14 September 2020

If you are on this site, you might be aware of some of the open source image processing and analysis tools are available to you. The toolbox in this space is rapidly expanding. But that doesn’t always mean it’s easy to navigate – it can actually be quite daunting. Luckily the bio-imaging community is friendly

LSFM series – Surfing on the data freak wave! PART I: Knowing your turf, knowing your surf!

Posted by Elisabeth Kugler, on 5 September 2020

Here we present a series of five blog posts with tips and tricks about light sheet microscopy. 1. The basics of LSFM (Sept 2020) 2. Improving sample mounting (Oct 2020) 3. Calibration and Acquisition (Nov 2020) 4. Tailoring the data (Dec 2020) 5. What is next? AI and smarter than us LSFM (Jan 2021)

Primers on Microscopy for Biologists - Resolution

Posted by Nachiket Vartak, on 15 July 2020

Formal definitions of resolution refer to imaginary objects such as infinitely small sources of light. I will avoid those and instead try to provide a pragmatic explanation. Practically, the spatial resolution is the size of the smallest structure that can be distinguished in the light coming from a specimen. All sensors and components of the

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Microscopy-related articles from our journals

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples Development 2024 151: dev202307
filoVision – using deep learning and tip markers to automate filopodia analysis J Cell Sci 2024 137: jcs261274
C omputational tools for quantifying actin filament numbers, lengths, and bundling Biology Open 2024 13: bio.060267

Microscopy-related preprint highlights

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