Microscopy preprints – applications in biology

Here is a curated selection of preprints published recently. In this post, we share preprints that use microscopy tools to answer questions in biology.

Correlative single molecule lattice light sheet imaging reveals the dynamic relationship between nucleosomes and the local chromatin environment
Timothy A. Daugird, Yu Shi, Katie L. Holland, Hosein Rostamian, Zhe Liu, Luke D. Lavis, Joseph Rodriguez, Brian D. Strahl, Wesley R. Legant

Probing the in-situ volumes of Arabidopsis leaf plastids using 3D confocal and scanning electron microscopy
Jan Knoblauch, Rainer Waadt, Asaph B. Cousins, Hans-Henning Kunz

Volume Electron Microscopy Reveals Unique Laminar Synaptic Characteristics in the Human Entorhinal Cortex
Sergio Plaza-Alonso, Nicolás Cano-Astorga, Javier DeFelipe, Lidia Alonso-Nanclares

Mechanical positive feedback and biochemical negative feedback combine to generate complex contractile oscillations in cytokinesis
Michael E. Werner, Dylan D. Ray, Coleman Breen, Michael F. Staddon, Florian Jug, Shiladitya Banerjee, Amy Shaub Maddox

Endoplasmic reticulum exit sites are segregated for secretion based on cargo size
Sonashree Saxena, Ombretta Foresti, Aofei Liu, Stefania Androulaki, Maria Pena Rodriguez, Ishier Raote, Bianxiao Cui, Meir Aridor, Vivek Malhotra

β-H-Spectrin is a key component of an apical-medial hub of proteins during cell wedging in tube morphogenesis
Ghislain Gillard, Katja Röper

A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction
Xiao Li, Rico Gamuyao, Ming-Lun Wu, Woo Jung Cho, Nathan B. Kurtz, Sharon V. King, R.A. Petersen, Daniel R. Stabley, Caleb Lindow, Leslie Climer, Abbas Shirinifard, Francesca Ferrara, Robert E. Throm, Camenzind G. Robinson, Alex Carisey, Alison G. Tebo, Chi-Lun Chang

Human dynein-dynactin is a fast processive motor in living cells
Vikash Verma, Patricia Wadsworth, Thomas J. Maresca

CryoET reveals actin filaments within platelet microtubules
Chisato Tsuji, Marston Bradshaw, Megan Allen, Molly L. Jackson, Judith Mantell, Ufuk Borucu, Alastair W. Poole, Paul Verkade, Ingeborg Hers, Danielle M. Paul, Mark P. Dodding

Dynamic label-free analysis of SARS-CoV-2 infection reveals virus-induced subcellular remodeling
Nell Saunders, Blandine Monel, Nadège Cayet, Lorenzo Archetti, Hugo Moreno, Alexandre Jeanne, Agathe Marguier, Timothy Wai, Olivier Schwartz, Mathieu Fréchin

Formation of recurring transient Ca2+-based intercellular communities during Drosophila hematopoiesis
Saar Ben David, Kevin Y.L. Ho, Guy Tanentzapf, Assaf Zaritsky

2D and 3D multiplexed subcellular profiling of nuclear instability in human cancer
Shannon Coy, Brian Cheng, Jong Suk Lee, Rumana Rashid, Lindsay Browning, Yilin Xu, Sankha S. Chakrabarty, Clarence Yapp, Sabrina Chan, Juliann B. Tefft, Emily Scott, Alexander Spektor, Keith L. Ligon, Gregory J. Baker, David Pellman, Peter K. Sorger, Sandro Santagata

Comprehensive mapping of sensory and sympathetic innervation of the developing kidney
Pierre-Emmanuel Y. N’Guetta, Sarah R. McLarnon, Adrien Tassou, Matan Geron, Grégory Scherrer, Lori L. O’Brien

Digitalized organoids: integrated pipeline for 3D high-speed analysis of organoid structures using multilevel segmentation and cellular topology
Hui Ting Ong, Esra Karatas, Gianluca Grenci, Florian Dilasser, Saburnisha Binte Mohamad Raffi, Damien Blanc, Titouan Poquillon, Elise Drimaracci, Dimitri Mikec, Cora Thiel, Oliver Ullrich, Victor Racine, Anne Beghin

Fast imaging of millimeter-scale areas with beam deflection transmission electron microscopy
Zhihao Zheng, Christopher S. Own, Adrian A. Wanner, Randal A. Koene, Eric W. Hammerschmith, William M. Silversmith, Nico Kemnitz, Ran Lu, David W. Tank, H. Sebastian Seung

High-speed Auto-Polarization Synchronization Modulation Three-dimensional Structured Illumination Microscopy
Yaning Li, Ruijie Cao, Wei Ren, Yunzhe Fu, Yiwei Hou, Suyi Zhong, Karl Zhanghao, Meiqi Li, Peng Xi

Transcranial cortex-wide imaging of murine ischemic perfusion with large-field multifocal illumination fluorescence microscopy
Zhenyue Chen, Quanyu Zhou, Jeanne Droux, Yu-Hang Liu, Chaim Glück, Irmak Gezginer, Matthias Wyss, Hikari AI Yoshihara, Diana Rita Kindler, Bruno Weber, Susanne Wegener, Mohamad El Amki, Daniel Razansky

Increased multiplexity in optical tissue clearing-based 3D immunofluorescence microscopy of the tumor microenvironment by LED photobleaching
Jingtian Zheng, Yi-Chien Wu, Evan H. Phillips, Xu Wang, Steve Seung-Young Lee

3D electron microscopy of the Leishmania mexicana cell cycle: Patterns of organelle duplication and segregation and their implications for parasite biology
Molly Hair, Ryuji Yanase, Flávia Moreira-Leite, Richard Wheeler, Jovana Sádlová, Petr Volf, Sue Vaughan, Jack Sunter

Microtubule length correlates with spindle length in C. elegans meiosis
Vitaly Zimyanin, Stefanie Redemann

Intermittent cell division dynamics in regenerating Arabidopsis roots reveals complex long-range interactions
T. Fallesen, S. Amarteifio, G. Pruessner, H. J. Jensen, G. Sena

Structure, interaction, and nervous connectivity of beta cell primary cilia
Andreas Müller, Nikolai Klena, Song Pang, Leticia Elizabeth Galicia Garcia, Davud Sulaymankhil, Oleksandra Topcheva, Monika Seliskar, Hassan Mziaut, Eyke Schöniger, Daniela Friedland, Nicole Kipke, Susanne Kretschmar, Carla Münster, Jürgen Weitz, Marius Distler, Thomas Kurth, Deborah Schmidt, Harald F. Hess, C. Shan Xu, Gaia Pigino, Michele Solimena

CD56/NCAM mediates cell migration of human NK cells by promoting integrin-mediated adhesion turnover
Amera L. Martinez, Michael J. Shannon, Tyler Sloan, Emily M. Mace

Cytokinetic abscission requires actin-dependent microtubule severing
Tamara Advedissian, Stéphane Frémont, Arnaud Echard

A Novel Imaging Method (FIM-ID) Reveals that Myofibrillogenesis Plays a Major Role in the Mechanically Induced Growth of Skeletal Muscle
Kent W Jorgenson, Jamie E Hibbert, Ramy KA Sayed, Anthony N Lange, Joshua S Godwin, Paulo HC Mesquita, Bradly A Ruple, Mason C McIntosh, Andreas N Kavazis, Michael D Roberts, Troy A Hornberger

Spatial and Temporal Coordination of Force-generating Actin-based Modules Drives Membrane Remodeling In Vivo
Marco Heydecker, Akiko Shitara, Desu Chen, Duy Tran, Andrius Masedunskas, Muhibullah Tora, Seham Ebrahim, Mark A. Appaduray, Jorge Luis Galeano Niño, Abhishek Bhardwaj, Kedar Narayan, Edna C. Hardeman, Peter W. Gunning, Roberto Weigert

Characterisation and correction of polarisation effects in fluorescently labelled fibres
Nandini Aggarwal, Richard Marsh, Stefania Marcotti, Tanya J Shaw, Brian Stramer, Susan Cox, Siân Culley

Orientation-Independent-DIC imaging reveals that a transient rise in macromolecular crowding contributes to mitotic chromosome condensation
Shiori Iida, Satoru Ide, Sachiko Tamura, Tomomi Tani, Tatsuhiko Goto, Michael Shribak, Kazuhiro Maeshima

3D-Imaging of synapses in neuronal tissues with synchrotron X-ray ptychography
Carles Bosch, Ana Diaz, Mirko Holler, Manuel Guizar-Sicairos, Tomas Aidukas, Alexandra Pacureanu, Elisabeth Müller, Christopher J. Peddie, Lucy Collinson, Yuxin Zhang, Andreas Menzel, Adrian A. Wanner, Andreas T. Schaefer

High-resolution ultrastructure and morphology of marine ammonia-oxidizing archaea Nitrosopumilus maritimus SCM1 revealed by Cryo-ET and STEM-EDS
Yangkai Zhou, An Yan, Jiawen Yang, Wei He, Shuai Guo, Yifan Li, Yanchao Dai, Xijiang Pan, Dongyu Cui, Wenkai Teng, Olivier Pereira, Ran Bi, Songze Chen, Lu Fan, Peiyi Wang, Yan Liao, Wei Qin, Sen-Fang Sui, Yuanqing Zhu, Chuanlun Zhang, Zheng Liu

Rapid Vacuolar Sorting of the Borate Transporter BOR1 Requires the Adaptor Protein Complex AP-4 in Arabidopsis
Akira Yoshinari, Yutaro Shimizu, Takuya Hosokawa, Akihiko Nakano, Tomohiro Uemura, Junpei Takano

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