Microscopy preprints – applications in biology

Posted by FocalPlane, on 26 January 2024

Here is a curated selection of preprints published recently. In this post, we share preprints that use microscopy tools to answer questions in biology.

Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag
Ellen H. Brumbaugh-Reed, Kazuhiro Aoki, Jared E. Toettcher

Figure extracted from Brumbaugh-Reed, *et al..* The image is made available under a CC-BY-NC 4.0 International license.

TRPV4 is expressed by enteric glia and muscularis macrophages of the colon but does not play a prominent role in colonic motility
Pradeep Rajasekhar, Simona E Carbone, Stuart T Johnston, Cameron J Nowell, Lukasz Wiklendt, Edmund J Crampin, Yinghan She, Jesse J DiCello, Ayame Saito, Luke Sorensen, Thanh Nguyen, Kevin MC Lee, John A Hamilton, Sebastian K King, Emily M Eriksson, Nick J Spencer, Brian D Gulbransen, Nicholas A Veldhuis, Daniel P Poole

Uncovering kinesin dynamics in neurites with MINFLUX
Jan Otto Wirth, Eva-Maria Schentarra, Lukas Scheiderer, Victor Macarrón-Palacios, Miroslaw Tarnawski, Stefan W. Hell

Dynamics and plasticity of stem cells in the regenerating human colonic epithelium
Koen C. Oost, Maurice Kahnwald, Silvia Barbiero, Gustavo de Medeiros, Simon Suppinger, Veronique Kalck, Ludivine Challet Meylan, Sebastien A. Smallwood, Michael B. Stadler, Prisca Liberali

Stimulation of NCAM1-14.3.3.ζδ-derived Peptide Interaction Fuels Angiogenesis and Osteogenesis in Ageing
Taha Kadir Yesin, Hanyu Liu, Zhangfan Ding, Amit Singh, Qi Tian, Yuheng Zhang, Biswajyoti Borah, Junyu Chen, Anjali P. Kusumbe

Figure extracted from Kadir Yesin, *et al..* The image is made available under a CC-BY 4.0 International license.

Microvilli regulate the release modes of alpha-tectorin to organize the domain-specific matrix architecture of the tectorial membrane
Ava Niazi, Ju Ang Kim, Dong-Kyu Kim, Di Lu, Igal Sterin, Joosang Park, Sungjin Park

The actin-spectrin submembrane scaffold restricts endocytosis along proximal axons
Florian Wernert, Satish Moparthi, Jeanne Lainé, Gilles Moulay, Fanny Boroni-Rueda, Florence Pelletier, Nicolas Jullien, Sofia Benkhelifa-Ziyat, Marie-Jeanne Papandréou, Christophe Leterrier, Stéphane Vassilopoulos

Volume Electron Microscopy Reveals Unique Laminar Synaptic Characteristics in the Human Entorhinal Cortex
Sergio Plaza-Alonso, Nicolas Cano-Astorga, Javier DeFelipe, Lidia Alonso-Nanclares

The Brain Image Library: A Community-Contributed Microscopy Resource for Neuroscientists
Mariah Kenney, Iaroslavna Vasylieva, Greg Hood, Ivan Cao-Berg, Luke Tuite, Rozita Laghaei, Alan M. Watson, Alexander J. Ropelewski

Continuous endosomes form functional subdomains and orchestrate rapid membrane trafficking in trypanosomes
Fabian Link, Alyssa Borges, Oliver Karo, Marvin Jungblut, Thomas Müller, Elisabeth Meyer-Natus, Timothy Krüger, Stefan Sachs, Nicola G. Jones, Mary Morphew, Markus Sauer, Christian Stigloher, J. Richard McIntosh, Markus Engstler

Structure, interaction, and nervous connectivity of beta cell primary cilia
Andreas Müller, Nikolai Klena, Song Pang, Leticia Elizabeth Galicia Garcia, Davud Sulaymankhil, Oleksandra Topcheva, Monika Seliskar, Hassan Mziaut, Eyke Schöniger, Daniela Friedland, Nicole Kipke, Susanne Kretschmar, Carla Münster, Jürgen Weitz, Marius Distler, Thomas Kurth, Deborah Schmidt, Harald F. Hess, C. Shan Xu, Gaia Pigino, Michele Solimena

A mechanosensitive channel Pkd2 modulates the assembly of myosin II and actin filaments in the cytokinetic contractile ring
Pritha Chowdhury, Debatrayee Sinha, Abhishek Poddar, Madhurya Chetluru, Qian Chen

The Septin Cytoskeleton is Required for Plasma Membrane Repair
M. Isabella Prislusky, Jonathan GT Lam, Viviana Ruiz Contreras, Marilynn Ng, Madeline Chamberlain, Sarika Pathak-Sharma, Madalyn Fields, Xiaoli Zhang, Amal O. Amer, Stephanie Seveau

Sorting of secretory proteins at the trans-Golgi network by human TGN46
Pablo Lujan, Carla Garcia-Cabau, Yuichi Wakana, Javier Vera Lillo, Carmen Rodilla-Ramírez, Hideaki Sugiura, Vivek Malhotra, Xavier Salvatella, Maria F. Garcia-Parajo, Felix Campelo

The membrane curvature inducing REEP1 proteins generate a novel ER-derived vesicular compartment
Yoko Shibata, Emily E. Mazur, Buyan Pan, Sebastien V. Hernandez, Jiuchun Zhang, Tom A. Rapoport

Photoswitchable endocytosis of biomolecular condensates in giant vesicles
Agustín Mangiarotti, Mina Aleksanyan, Macarena Siri, Reinhard Lipowsky, Rumiana Dimova

Figure extracted from Mangiarotti, *et al.* The image is made available under a CC-BY 4.0 International license.

Reversible assembly and disassembly of V-ATPase during the lysosome regeneration cycle
Ioana Sava, Luther J. Davis, Sally R. Gray, Nicholas A. Bright, J. Paul Luzio

Figure extracted from Sava, *et al..* The image is made available under a CC-BY 4.0 International license.

Fluidic shear stress alters clathrin dynamics and vesicle formation in endothelial cells
Tomasz J. Nawara, Elizabeth Sztul, Alexa L. Mattheyses

Figure extracted from Nawara, *et al.* The image is made available under a CC-BY 4.0 International license.

Liquid-liquid Phase Separation of Alpha-synuclein Increases the Structural Variability of Fibrils Formed during Amyloid Aggregation
Mantas Ziaunys, Darius Sulskis, Dominykas Veiveris, Aurimas Kopustas, Ruta Snieckute, Kamile Mikalauskaite, Andrius Sakalauskas, Marijonas Tutkus, Vytautas Smirnovas

Imaging the voltage of neurons distributed across entire brains of larval zebrafish
Zeguan Wang, Jie Zhang, Panagiotis Symvoulidis, Wei Guo, Lige Zhang, Matthew A. Wilson, Edward S. Boyden

In vivo optimized three-photon imaging of intact mouse tibia links plasma cell motility to functional states in the bone marrow
Asylkhan Rakhymzhan, Alexander F. Fiedler, Robert Günther, Scott Domingue, Laura Wooldridge, Ruth Leben, Yu Cao, Anne Bias, Jay Roodselaar, Ralf Köhler, Carolin Ulbricht, Judith Heidelin, Volker Andresen, Ingeborg Beckers, Astrid Haibel, Georg Duda, Anja E. Hauser, Raluca A. Niesner

Nanoscale profiling of evolving intermolecular interactions in ageing FUS condensates
Alyssa Miller, Zenon Toprakcioglu, Seema Qamar, Peter St. George-Hyslop, F. Simone Ruggeri, Tuomas P. J. Knowles, Michele Vendruscolo

Cryo-EM images of phase separated lipid bilayer vesicles analyzed with a machine learning approach
Karan D. Sharma, Milka Doktorova, M. Neal Waxham, Frederick A. Heberle

PIEZO1-HaloTag hiPSCs: Bridging Molecular, Cellular and Tissue Imaging
Gabriella A. Bertaccini, Elizabeth L. Evans, Jamison L. Nourse, George D. Dickinson, Gaoxiang Liu, Ignasi Casanellas, Sayan Seal, Alan T. Ly, Jesse R. Holt, Shijun Yan, Elliot E. Hui, Mitradas M. Panicker, Srigokul Upadhyayula, Ian Parker, Medha M. Pathak