Microscopy preprints – applications in biology

Here is a curated selection of preprints published recently. In this post, we share preprints, released before 24 May, that use microscopy tools to answer questions in biology.

Force-activated zyxin assemblies coordinate actin nucleation and crosslinking to orchestrate stress fiber repair
Donovan Y.Z. Phua, Xiaoyu Sun, Gregory M. Alushin

Improved whole-mount immunofluorescence protocol for consistent and robust labeling of adult Drosophila melanogaster adipose tissue
Rachael K. Ott, Alissa R. Armstrong

A genetic and microscopy toolkit for live imaging of whole-body regeneration in Macrostomum lignano
R. Nelson Hall, Hongquan Li, Chew Chai, Sidney Vermeulen, Robin R. Bigasin, Eun Sun Song, Jesse Gibson, Manu Prakash, Andrew Z. Fire, Bo Wang

Temporal and anteriorly positioned mitotic zones drive asymmetric microtubule patterns needed for Left-Right Organizer development
Yan Wu, Yiling Lan, Favour Ononiwu, Abigail Poole, Kirsten Rasmussen, Jonah Da Silva, Abdalla Wael Shamil, Li-En Jao, Heidi Hehnly

Compartmentalized Cytoplasmic Flows Direct Protein Transport to the Cell’s Leading Edge
Catherine G. Galbraith, Brian P. English, Ulrike Boehm, James A. Galbraith

Topology changes of the regenerating Hydra define actin nematic defects as mechanical organizers of morphogenesis
Yamini Ravichandran, Matthias Vogg, Karsten Kruse, Daniel JG Pearce, Aurélien Roux

Pattern formation along signaling gradients driven by active droplet behaviour of cell groups
Hugh Z Ford, Giulia L Celora, Elizabeth R Westbrook, Mohit P Dalwadi, Benjamin J Walker, Hella Baumann, Cornelis J. Weijer, Philip Pearce, Jonathan R Chubb

Molecular architecture of glideosome and nuclear F-actin in Plasmodium falciparum
Vojtech Pražák, Daven Vasishtan, Kay Grünewald, Ross G. Douglas, Josie L. Ferreira

Quantitative imaging of species-specific lipid transport in mammalian cells
Juan M. Iglesias-Artola, Kai Schuhmann, Kristin Böhlig, H. Mathilda Lennartz, Milena Schuhmacher, Pavel Barahtjan, Cristina Jiménez López, Radek Šachl, Karina Pombo-Garcia, Annett Lohmann, Petra Riegerová, Martin Hof, Björn Drobot, Andrej Shevchenko, Alf Honigmann, André Nadler

Crosstalk of growth factor receptors at plasma membrane clathrin-coated sites
Marco A. Alfonzo-Méndez, Marie-Paule Strub, Justin W. Taraska

Expansion of in vitro Toxoplasma gondii cysts using enzymatically enhanced ultrastructure expansion microscopy
Kseniia Bondarenko, Floriane Limoge, Kayvon Pedram, Mathieu Gissot, Joanna C. Young

Correlative multiscale 3D imaging of mouse primary and metastatic tumors by sequential light sheet and confocal fluorescence microscopy
Jingtian Zheng, Yi-Chien Wu, Xiaoying Cai, Philana Phan, Ekrem Emrah Er, Zongmin Zhao, Steve Seung-Young Lee

Deep learning based reconstruction of embryonic cell-division cycle from label-free microscopy time-series of evolutionarily diverse nematodes
Dhruv Khatri, Chaitanya A. Athale

Mosquito Tissue Ultrastructure-Expansion Microscopy (MoTissU-ExM) enables ultrastructural and anatomical analysis of malaria parasites and their mosquito
Benjamin Liffner, Thiago Luiz Alves e Silva, Joel Vega-Rodriguez, Sabrina Absalon

Live-cell single-molecule fluorescence microscopy for protruding organelles reveals regulatory mechanisms of MYO7A-driven cargo transport in stereocilia of inner ear hair cells
Takushi Miyoshi, Harshad D. Vishwasrao, Inna A. Belyantseva, Mrudhula Sajeevadathan, Yasuko Ishibashi, Samuel M. Adadey, Narinobu Harada, Hari Shroff, Thomas B. Friedman

Real-time label-free exploration of the dynamics and interactions of bacteriophages
Francesco Giorgi, Judith M. Curran, Liberty Duignan, Joanne L. Fothergill, Sam Chenery, Eann A. Patterson

Long-term imaging of three-dimensional hyphal development using the ePetri dish
Oumeng Zhang, Nic Dahlquist, Zachary Leete, Michael Xu, Dean Schneider, Changhuei Yang

Detailing organelle division and segregation in Plasmodium falciparum
Julie M.J. Verhoef, Cas Boshoven, Felix Evers, Laura J. Akkerman, Barend C.A. Gijsbrechts, Marga van de Vegte-Bolmer, Geert-Jan van Gemert, Akhil B. Vaidya, Taco W.A. Kooij

Cell morphogenesis via self-propelled treadmilling actin waves
Kio Yagami, Kentarou Baba, Takunori Minegishi, Shinji Misu, Hiroko Katsuno-Kambe, Kazunori Okano, Yuichi Sakumura, Yoichiroh Hosokawa, Naoyuki Inagaki

Live-cell imaging and CLEM reveal the existence of ACTN4-dependent ruffle-edge lamellipodia acting as a novel mode of cell migration
Haruka Morishita, Katsuhisa Kawai, Youhei Egami, Kazufumi Honda, Nobukazu Araki

Thrombospondins 1 and 4 undergo coordinated transport to multicore cytotoxic granules to regulate SMAP biogenesis and function in CTL-mediated cytotoxicity
Chiara Cassioli, Nagaja Capitani, Claire C. Staton, Claudia Schirra, Francesca Finetti, Anna Onnis, Nadia Alawar, Szu-Min Tu, Ludovica Lopresti, Vanessa Tatangelo, Carmela Tangredi, Salvatore Valvo, Hsin-Fang Chang, Annachiara Miccoli, Ewaldus B. Compeer, Jemma Nicholls, Bruce Blazar, Giuseppe Marotta, Matthew J. A. Wood, Livio Trentin, Laura Patrussi, Michael L. Dustin, Ute Becherer, Cosima T. Baldari

Noninvasive time-lapse 3D subcellular analysis of embryo development for machine learning-enabled prediction of blastocyst formation
Chungha Lee, Geon Kim, Taeseop Shin, Sangho Lee, Jae Young Kim, Kyoung Hee Choi, Jieun Do, Jaehyeong Park, Jaephil Do, Ji Hyang Kim, YongKeun Park

A Role for Cross-linking Proteins in Actin Filament Network Organization and Force Generation
Jennifer M. Hill, Songlin Cai, Michael D. Carver, David G. Drubin

Pushed to the edge: hundreds of myosin 10s pack into filopodia and could cause traffic jams on actin
Julia Shangguan, Ronald S. Rock

Volumetric analysis of the terminal ductal lobular unit architecture and cell phenotypes in the human breast
Oona Paavolainen, Markus Peurla, Leena M Koskinen, Jonna Pohjankukka, Kamyab Saberi, Ella Tammelin, Suvi-Riitta Sulander, Masi Valkonen, Larissa Mourao, Pia Boström, Nina Brück, Pekka Ruusuvuori, Colinda LGJ Scheele, Pauliina Hartiala, Emilia Peuhu

Cortico-amygdala synaptic structural abnormalities produced by templated aggregation of α-synuclein
Nolwazi Z. Gcwensa, Dreson L. Russell, Khaliah Y. Long, Charlotte F. Brzozowski, Xinran Liu, Karen L. Gamble, Rita M. Cowell, Laura A. Volpicelli-Daley

Metabolic heterogeneity of colorectal cancer as a prognostic factor: insights gained from fluorescence lifetime imaging
Anastasia D Komarova, Snezhana D Sinyushkina, Ilia D Shchechkin, Irina N Druzhkova, Sofia A Smirnova, Vitaliy M Terekhov, Artem M Mozherov, Nadezhda I Ignatova, Elena E Nikonova, Evgeny A Shirshin, Liubov E Shimolina, Sergey V Gamayunov, Vladislav I Shcheslavskiy, Marina V Shirmanova

The IQGAP-related RasGAP IqgC regulates cell-substratum adhesion in Dictyostelium discoideum
Lucija Mijanović, Darija Putar, Lucija Mimica, Sabina Klajn, Vedrana Filić, Igor Weber

Deep-Tissue Spatial Omics: Imaging Whole-Embryo Transcriptomics and Subcellular Structures at High Spatial Resolution
Valentina Gandin, Jun Kim, Liang-Zhong Yang, Yumin Lian, Takashi Kawase, Amy Hu, Konrad Rokicki, Greg Fleishman, Paul Tillberg, Alejandro Aguilera Castrejon, Carsen Stringer, Stephan Preibisch, Zhe J. Liu

A 3D atlas of sexually dimorphic lumbosacral motor neurons that control and integrate pelvic visceral and somatic functions in rats
John-Paul Fuller-Jackson, Ziying Yang, Nicole M Wiedmann, Alan Watson, Nathaniel EC Jenkins, Janet R Keast, Peregrine B Osborne

BMP4 regulates asymmetric Pkd2 distribution in mouse nodal immotile cilia and ciliary mechanosensing required for left–right determination
Takanobu A. Katoh, Tim Lange, Yoshiro Nakajima, Kenta Yashiro, Yasushi Okada, Hiroshi Hamada

Cardiolipin clustering promotes mitochondrial membrane dynamics
Kelly E. Zuccaro, Luciano Abriata, Fernando Meireles, Frank R. Moss III, Adam Frost, Matteo Dal Peraro, Halil Aydin

Harnessing homeostatically active RhoC at cell junctions preserves human endothelial barrier function during inflammation
Natalia Colás-Algora, Pablo Muñoz-Pinillos, Susana Barroso, Cristina Cacho-Navas, Álvaro Caballero, Gema Cerro-Tello, Gema de Rivas, Martín González-Fernández, Ignacio Jiménez-Alfaro, Manuel Fresno, Catalina Ribas, Alberto Paradela, Eduardo López-Collazo, José Jesús Fernández, Jaime Millán

A delta-tubulin/epsilon-tubulin/Ted protein complex is required for centriole architecture
Rachel Pudlowski, Lingyi Xu, Ljiljana Milenkovic, Katherine Hemsworth, Tim Stearns, Jennifer T. Wang

Molecular counting of myosin force generators in growing filopodia
Gillian N. Fitz, Matthew J. Tyska

High-speed imaging of giant unilamellar vesicle formation in cDICE
Lori Van de Cauter, Yash K. Jawale, Daniel Tam, Lucia Baldauf, Lennard van Buren, Gijsje H. Koenderink, Marileen Dogterom, Kristina A. Ganzinger

Imaging the extracellular matrix in live tissues and organisms with a glycan-binding fluorophore
Antonio Fiore, Guoqiang Yu, Jason J. Northey, Ronak Patel, Thomas A. Ravenscroft, Richard Ikegami, Wiert Kolkman, Pratik Kumar, Jonathan B. Grimm, Tanya L. Dilan, Virginia M.S. Ruetten, Misha B. Ahrens, Hari Shroff, Luke D. Lavis, Shaohe Wang, Valerie M. Weaver, Kayvon Pedram

Super-Resolution Imaging Uncovers Nanoscale Tau Aggregate Hyperphosphorylation Patterns in Human Alzheimer’s Disease Brain Tissue
Adriana N. Santiago-Ruiz, Siewert Hugelier, Charles R. Bond, Edward B. Lee, Melike Lakadamyali

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