Microscopy preprints – new tools and techniques in imaging

Posted by FocalPlane, on 9 August 2024

Here is a curated selection of preprints posted recently on new tools and techniques in imaging. Let us know if we are missing any recent preprints that are on your reading list!

Array Detection Enables Large Localization Range for Simple and Robust MINFLUX
Eli Slenders, Sanket Patil, Marcus Oliver Held, Alessandro Zunino, Giuseppe Vicidomini

Lipid-directed covalent fluorescent labeling of plasma membranes for long-term imaging, barcoding and manipulation of cells
Nathan Aknine, Remi Pelletier, Andrey S. Klymchenko

Figure extracted from Aknine, *et al.* made available under a CC-BY 4.0 International license.

The TissueTractor, a device for applying large strains to tissues and cells for simultaneous high-resolution live cell microscopy
Jing Yang, Emily Hearty, Yingli Wang, Deepthi S. Vijayraghavan, Timothy Walter, Sommer Anjum, Carsten Stuckenholz, Ya-Wen Cheng, Sahana Balasubramanian, Adam V. Kwiatkowski, Lance A. Davidson

Fully-Automated Multicolour Structured Illumination Module for Super-resolution Microscopy
Haoran Wang, Peter T. Brown, Jessica Ullom, Douglas P. Shepherd, Rainer Heintzmann, Benedict Diederich

Figure extracted from Wang, *et al.* made available under a CC-BY 4.0 International license.

Single-molecule detection and super-resolution imaging with a portable and adaptable 3D-printed microscopy platform (Brick-MIC)
Gabriel G. Moya Muñoz, Oliver Brix, Philipp Klocke, Paul D. Harris, Jorge R. Luna Piedra, Nicolas D. Wendler, Eitan Lerner, Niels Zijlstra, Thorben Cordes

Figure extracted from Moya Muñoz, *et al.* made available under a CC-BY-NC 4.0 International license.

Obtaining super-resolved images at the mesoscale through Super-Resolution Radial Fluctuations
Mollie Brown, Shannan Foylan, Liam M. Rooney, Gwyn W. Gould, Gail McConnell

Multispectral live-cell imaging with uncompromised spatiotemporal resolution
Akaash Kumar, Kerrie E. McNally, Yuexuan Zhang, Alex Haslett-Saunders, Xinru Wang, Jordi Guillem-Marti, David Lee, Buwei Huang, Robert R. Kay, David Baker, Emmanuel Derivery, James D. Manton

Figure extracted from Kumar, *et al.* made available under a CC-BY 4.0 International license.

Coordinate-based neural representations for computational adaptive optics in widefield microscopy
Iksung Kang, Qinrong Zhang, Stella X. Yu, Na Ji

A versatile platform for single fluorescent protein-based fluorescence lifetime biosensors
Chongxia Zhong, Satoshi Arai, Yasushi Okada

MINFLUX fluorescence nanoscopy in biological tissue
Thea Moosmayer, Kamila A. Kiszka, Volker Westphal, Jasmin K. Pape, Marcel Leutenegger, Heinz Steffens, Seth G. N. Grant, Steffen J. Sahl, Stefan W. Hell

Tailoring the self-blinking of sulfonamide rhodamine for long-term protein-localizing super-resolution imaging
Xue Zhang, Ying Zheng, Lujia Yang, Zhiwei Ye, Yi Xiao

PEPCy: Photostable fluoromodules for live cell, super-resolution microscopy of surface proteins
Moeka Sasazawa, Afroze Chimthanawala, Rui Zeng, Danah Kim, Katherine Buchan, Ming Zhang, Saumya Saurabh

Super-resolution imaging of proteins inside live mammalian cells with mLIVE-PAINT
Haresh Bhaskar, Zoe Gidden, Gurvir Virdi, Dirk-Jan Kleinjan, Susan J. Rosser, Sonia Gandhi, Lynne Regan, Mathew H. Horrocks

Surpassing Light Inhomogeneities in Structured-Illumination Microscopy with FlexSIM
Emmanuel Soubies, Alejandro Nogueron, Florence Pelletier, Thomas Mangeat, Christophe Leterrier, Michael Unser, Daniel Sage

Nonlinear sound-sheet microscopy: imaging opaque organs at the capillary and cellular scale
Baptiste Heiles, Flora Nelissen, Dion Terwiel, Byung Min Park, Eleonora Munoz Ibarra, Agisilaos Matalliotakis, Rick Waasdorp, Tarannum Ara, Pierina Barturen-Larrea, Mengtong Duan, Mikhail G. Shapiro, Valeria Gazzola, David Maresca

Development of AI-assisted microscopy frameworks through realistic simulation in pySTED
Anthony Bilodeau, Albert Michaud-Gagnon, Julia Chabbert, Benoit Turcotte, Jörn Heine, Audrey Durand, Flavie Lavoie-Cardinal

QCM: real-time quantitative quality control of single-molecule localization microscopy acquisitions
Sébastien Mailfert, Meriem Djendli, Roxane Fabre, Didier Marguet, Nicolas Bertaux

Figure extracted from Mailfert, *et al.* made available under a CC-BY-NC 4.0 International license.

Vortex light field microscopy: 3D spectral single-molecule imaging with a twist
Boya Zhang, Sam Daly, Chengxi Zhu, Martin O. Lenz, Lucien E. Weiss, Lisa-Maria Needham, Ruby Peters, Steven F. Lee, Kevin O’Holleran

Expansion-assisted selective plane illumination microscopy for nanoscale imaging of centimeter-scale tissues
Adam Glaser, Jayaram Chandrashekar, Sonya Vasquez, Cameron Arshadi, Naveen Ouellette, Xiaoyun Jiang, Judith Baka, Gabor Kovacs, Micah Woodard, Sharmishtaa Seshamani, Kevin Cao, Nathan Clack, Andrew Recknagel, Anna Grim, Pooja Balaram, Emily Turschak, Marcus Hooper, Alan Liddell, John Rohde, Ayana Hellevik, Kevin Takasaki, Lindsey Erion Barner, Molly Logsdon, Chris Chronopoulos, Saskia de Vries, Jonathan Ting, Steve Perlmutter, Brian Kalmbach, Nikolai Dembrow, Bosiljka Tasic, R. Clay Reid, David Feng, Karel Svoboda

PhotoFiTT: A Quantitative Framework for Assessing Phototoxicity in Live-Cell Microscopy Experiments
Mario Del Rosario, Estibaliz Gómez-de-Mariscal, Leonor Morgado, Raquel Portela, Guillaume Jacquemet, Pedro M. Pereira, Ricardo Henriques

Figure extracted from Del Rosario, *et al.* made available under a CC-BY 4.0 International license.

ExPOSE: A comprehensive toolkit to perform expansion microscopy in plant protoplast systems
Kevin L. Cox Jr., Sarah A. Pardi, Lily O’Connor, Anastasiya Klebanovych, David Huss, Dmitri A. Nusinow, Blake C. Meyers, Kirk J. Czymmek

Deep learning-based aberration compensation improves contrast and resolution in fluorescence microscopy
Min Guo, Yicong Wu, Chad M. Hobson, Yijun Su, Shuhao Qian, Eric Krueger, Ryan Christensen, Grant Kroeschell, Johnny Bui, Matthew Chaw, Lixia Zhang, Jiamin Liu, Xuekai Hou, Xiaofei Han, Zhiye Lu, Xuefei Ma, Alexander Zhovmer, Christian Combs, Mark Moyle, Eviatar Yemini, Huafeng Liu, Zhiyi Liu, Alexandre Benedetto, Patrick La Riviere, Daniel Colón-Ramos, Hari Shroff

Extended-depth of field random illumination microscopy, EDF-RIM, provides super-resolved projective imaging
Lorry Mazzella, Thomas Mangeat, Guillaume Giroussens, Benoit Rogez, Hao Li, Justine Creff, Mehdi Saadaoui, Carla Martins, Ronan Bouzignac, Simon Labouesse, Jérome Idier, Frédéric Galland, Marc Allain, Anne Sentenac, Loïc LeGoff

A novel method (RIM-Deep) enhances imaging depth and resolution stability of deep-cleared brain tissue in inverted confocal microscopy
Yisi Liu, Pu Wang, Junjie Zou, Hongwei Zhou

Figure extracted from Liu, *et al.* made available under a CC-BY 4.0 International license.

3D micropatterned traction force microscopy: a technique to control three-dimensional cell shape while measuring cell-substrate force transmission
Laura M. Faure, Manuel Gómez-González, Ona Baguer, Jordi Comelles, Elena Martínez, Marino Arroyo, Xavier Trepat, Pere Roca-Cusachs

Wide-field bond-selective fluorescence imaging: from single-molecule to cellular imaging beyond video-rate
Dongkwan Lee, Haomin Wang, Philip A. Kocheril, Xiaotian Bi, Noor Naji, Lu Wei

An Omni-Mesoscope for multiscale high-throughput quantitative phase imaging of cellular dynamics and high-content molecular characterization
Hongqiang Ma, Maomao Chen, Jianquan Xu, Yongxin Zhao, Yang Liu

High-speed three-dimensional random access scanning with SPARCLS
Caroline Berlage, Urs L. Böhm, Ana Sanchez Moreno, Julia Ledderose, Albert Gidon, Matthew E. Larkum, Andrew Plested, Benjamin Judkewitz

Nanoscale volumetric fluorescence imaging via photochemical sectioning
Wei Wang, Xiongtao Ruan, Gaoxiang Liu, Daniel E. Milkie, Wenping Li, Eric Betzig, Srigokul Upadhyayula, Ruixuan Gao

Multi-species cryoEM calibration and workflow verification standard
Daija Bobe, Mykhailo Kopylov, Jessalyn Miller, Aaron P. Owji, Edward T. Eng

BOOST: a robust ten-fold expansion method on hour-scale
Jinyu Guo, Hui Yang, Chixiang Lu, Di Cui, Murong Zhao, Cun Li, Weihua Chen, Qian Yang, Zhijie Li, Mingkun Chen, Shanchao Zhao, Jie Zhou, Jiaye He, Haibo Jiang

Figure extracted from Guo, *et al.* made available under a CC-BY-NC 4.0 International license.

Cryo-electron tomography pipeline for plasma membranes
Willy W. Sun, Dennis J. Michalak, Kem A. Sochacki, Prasanthi Kunamaneni, Marco A. Alfonzo-Méndez, Andreas M. Arnold, Marie-Paule Strub, Jenny E. Hinshaw, Justin W. Taraska

Long axial-range double-helix point spread functions for 3D volumetric super-resolution imaging
Yuya Nakatani, Scott Gaumer, Yoav Shechtman, Anna-Karin Gustavsson

Thickness and quality controlled fabrication of fluorescence-targeted frozen-hydrated lamellae
Daan B. Boltje, Radim Skoupy, Clémence Taisne, Wiel H. Evers, Arjen J. Jakobi, Jacob P. Hoogenboom