Microscopy preprints – applications in biology

Posted by FocalPlane, on 23 August 2024

Here is a curated selection of preprints published recently. In this post, we share preprints that use microscopy tools to answer questions in biology.

Myosin forces elicit an F-actin structural landscape that mediates mechanosensitive protein recognition
Ayala G. Carl, Matthew J. Reynolds, Pinar S. Gurel, Donovan Y.Z. Phua, Xiaoyu Sun, Lin Mei, Keith Hamilton, Yasuharu Takagi, Alex J. Noble, James R. Sellers, Gregory M. Alushin

Figure extracted from Carl, *et al.* made available under a CC-BY 4.0 International license.

Nanobody immunolabelling and three-dimensional imaging reveals spatially restricted LYVE1 expression by kidney lymphatic vessels in mice
Eva Maria Funk, Daniyal J Jafree, Nils Rouven Hansmeier, Clàudia Abad Baucells, Rose Yinghan Behncke, Gideon Pomeranz, Maria Kolatsi-Joannou, William J Mason, Dale Moulding, Lauren G Russell, Sascha Ulfers, Laura Wilson, David A Long, René Hägerling

Figure extracted from Funk, *et al.* made available under a CC-BY 4.0 International license.

Subsets of extraocular motoneurons produce kinematically distinct saccades during hunting and exploration
Charles K. Dowell, Thomas Hawkins, Isaac H. Bianco

Figure extracted from Dowell, *et al.* made available under a CC-BY 4.0 International license.

Arp2/3 iso-complexes limit t-tubule overgrowth to ensure myofiber formation and synchronous contraction
Ana Raquel Pereira, Ana da Rosa Soares, Tianyang Liu, Josie Liane Ferreira, Graciano Leal, Inês Faleiro, Naoko Kogata, Michael Way, Carolyn A. Moores, Edgar R. Gomes

Super-resolution imaging of the neuronal cytoskeleton
Butler-Hallissey, Ciarán, Leterrier, Christophe

The axillary lymphoid organ – an external, experimentally accessible immune organ in the zebrafish
Daniel Castranova, Madeleine I. Kenton, Aurora Kraus, Christopher W. Dell, Jong S. Park, Marina Venero Galanternik, Gilseung Park, Daniel N. Lumbantobing, Louis Dye, Miranda Marvel, James Iben, Kiyohito Taimatsu, Van Pham, Reegan J. Willms, Lucas Blevens, Tanner F. Robertson, Yiran Hou, Anna Huttenlocher, Edan Foley, Lynne R. Parenti, J. Kimble Frazer, Kedar Narayan, Brant M. Weinstein

The actin cortex acts as a mechanical memory of morphology in confined migrating cells
Yohalie Kalukula, Marine Luciano, Guillaume Charras, David B. Brückner, Sylvain Gabriele

Mechanistic basis of temperature-adaptation in microtubule dynamics across frog species
Luca Troman, Ella de Gaulejac, Abin Biswas, Jennifer Stiens, Benno Kuropka, Carolyn Moores, Simone Reber

The Pollen Tube Penetrates the Synergid Cell by Formation of a Peritubular Membrane
Nicholas Desnoyer, Marta Belloli, Stefano Bencivenga, Philipp Denninger, Ueli Grossniklaus

Oxidative stress promotes axonal atrophy through alterations in microtubules and EB1 function
Samuel Shields, Oliver Wilkes, Illana Gozes, Natalia Sanchez-Soriano

Figure extracted from Shields, *et al.* made available under a CC-BY 4.0 International license.

Actin dynamics sustains spatial gradients of membrane tension in adherent cells
Juan Manuel García-Arcos, Amine Mehidi, Julissa Sánchez Velázquez, Pau Guillamat, Caterina Tomba, Laura Houzet, Laura Capolupo, Giovanni D’Angelo, Adai Colom, Elizabeth Hinde, Charlotte Aumeier, Aurélien Roux

Figure extracted from García-Arcos, *et al.* made available under a CC-BY 4.0 International license.

Multiscale mechanics drive functional maturation of the vertebrate heart
Toby G R Andrews, Jake Cornwall-Scoones, Marie-Christine Ramel, Kirti Gupta, James Briscoe, Rashmi Priya

Cytoplasmic ribosomes on mitochondria alter the local membrane environment for protein import
Ya-Ting Chang, Benjamin A. Barad, Hamidreza Rahmani, Brian M. Zid, Danielle A. Grotjahn

Reverse development in the ctenophore Mnemiopsis leidyi
Joan J. Soto-Angel, Pawel Burkhardt

Golgi compaction facilitates microtubule nucleation to drive adult vertebrate peripheral neuron regeneration
Alice E Mortimer, Adam J Reid, Raman M Das

Figure extracted from Mortimer, *et al.* made available under a CC-BY 4.0 International license.

A conserved regulation of cell expansion underlies notochord mechanics, spine morphogenesis, and endochondral bone lengthening
Brittney Voigt, Katherine Frazier, Donya Yazdi, Paul Gontarz, Bo Zhang, Diane S. Sepich, Lilianna Solnica-Krezel, Ryan S. Gray

Live-cell imaging and CLEM reveal the existence of ACTN4-dependent ruffle-edge lamellipodia acting as a novel mode of cell migration
Haruka Morishita, Katsuhisa Kawai, Youhei Egami, Kazufumi Honda, Nobukazu Araki

Cryo-electron tomography of the actin cytoskeleton and membrane organization during lamellipodia formation using optogenetics
Hironori Inaba, Tsuyoshi Imasaki, Kazuhiro Aoyama, Shogo Yoshihara, Hiroko Takazaki, Takayuki Kato, Hidemasa Goto, Kaoru Mitsuoka, Ryo Nitta, Takao Nakata

Time-resolved tracking of cellulose biosynthesis and microfibril network assembly during cell wall regeneration in live Arabidopsis protoplasts
Hyun Huh, Dharanidaran Jayachandran, Junhong Sun, Mohammad Irfan, Eric Lam, Shishir P. S. Chundawat, Sang-Hyuk Lee

Cdhr1a and pcdh15b link photoreceptor outer segments with inner segment calyceal processes revealing a potential mechanism for cone-rod dystrophy
Meet K. Patel, Warlen Piedade, Jakub K. Famulski

Figure extracted from Patel, *et al.* made available under a CC-BY 4.0 International license.

ARF1 compartments direct cargo flow via maturation into recycling endosomes
Alexander Stockhammer, Petia Adarska, Vini Natalia, Anja Heuhsen, Antonia Klemt, Gresy Bregu, Shelly Harel, Carmen Rodilla-Ramirez, Carissa Spalt, Ece Özsoy, Paula Leupold, Alica Grindel, Eleanor Fox, Joy Orezimena Mejedo, Amin Zehtabian, Helge Ewers, Dmytro Puchkov, Volker Haucke, Francesca Bottanelli

New imaging tools reveal live cellular collagen secretion, fibril dynamics and network organisation
Olivia Kent, Ellie R. Casey, Max Brown, Steven Bell, Matt Ehrman, Mike Flagler, Arto Määttä, Adam M. Benham, Timothy J. Hawkins

Single influenza A viruses induce nanoscale cellular reprogramming at the virus-cell interface
Lukas Broich, Hannah Wullenkord, Maria Kaukab Osman, Yang Fu, Peter Reuther, Mark Brönstrup, Christian Sieben

Depletion of endomembrane reservoirs drives phagocytic appetite exhaustion in macrophages
Aaron Fountain, Mélanie Mansat, Sierra Soffiaturo, Maria C. Gimenez, Serene Moussaoui, Maya Ezzo, Munira B. Verdawala, Karen Fung, Charlene Lancaster, Elliott Somerville, Boris Hinz, Mauricio R. Terebiznik, Roberto J. Botelho

Oxidative stress response mediated by the yeast Rho5 GTPase depends on the proper spatiotemporal distribution of its dimeric GEF
Linnet Bischof, Jürgen J. Heinisch

Ultrastructural diversity of alpha-Synuclein pathology in the post-mortem brain of Parkinson patients: implications for Lewy Body formation
Amanda J Lewis, Lukas van den Heuvel, Marta di Fabrizio, Domenic Burger, Evelien Huisman, John GJM Bol, Wilma DJ van de Berg, Henning Stahlberg

The initiation and early development of apical-basal polarity in Toxoplasma gondii
Luisa F. Arias Padilla, Jonathan Munera Lopez, Aika Shibata, John M. Murray, Ke Hu

3D-Imaging of synapses in neuronal tissues with synchrotron X-ray ptychography
Carles Bosch, Tomas Aidukas, Mirko Holler, Alexandra Pacureanu, Elisabeth Müller, Christopher J. Peddie, Yuxin Zhang, Phil Cook, Lucy Collinson, Oliver Bunk, Andreas Menzel, Manuel Guizar-Sicairos, Gabriel Aeppli, Ana Diaz, Adrian A. Wanner, Andreas T. Schaefer

Figure extracted from Bosch, *et al.* made available under a CC-BY 4.0 International license.

Intracellular Ebola Virus nucleocapsid assembly revealed by in situ cryo-electron tomography.
Reika Watanabe, Dawid Zyla, Diptiben Parekh, Connor Hong, Ying Jones, Sharon L. Schendel, Willian Wan, Guillaume Castillon, Erica Ollmann Saphire

Sequential replacement of PSD95 subunits in postsynaptic supercomplexes is slowest in the cortex
Katie Morris, Edita Bulovaite, Takeshi Kaizuka, Sebastian Schnorrenberg, Candace Adams, Noboru H. Komiyama, Lorena Mendive-Tapia, Seth G. N. Grant, Mathew H. Horrocks

DOCK8 regulates a mechanosensitive actin redistribution that maintains immune cell cohesion and protects the nucleus during migration
Connie Shen, Jérémy Postat, Aysha Cerf, Mauricio Merino, Aanya Bhagrath, Dhanesh Patel, Vincent Luo, Afnan Abu-Thuraia, Caitlin Schneider, Abhinav Sharma, Woong-Kyung Suh, Allen Ehrlicher, Jean-François Côté, Judith N. Mandl

Mapping the nuclear landscape with multiplexed super-resolution fluorescence microscopy
Fariha Rahman, Victoria Augoustides, Emma Tyler, Timothy A. Daugird, Christian Arthur, Wesley R. Legant

Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopy
Mitsuhiro Abe, Masataka Yanagawa, Michio Hiroshima, Toshihide Kobayashi, Yasushi Sako

Enhanced Visualization of Influenza A Virus Entry Using Virus-View Atomic Force Microscopy
Aiko Yoshida, Yoshitsugu Uekusa, Takeshi Suzuki, Michael Bauer, Nobuaki Sakai, Yohei Yamauchi

3D Reconstruction of Neuronal Allometry and Neuromuscular Projections in Asexual Planarians Using Expansion Tiling Light Sheet Microscopy
Jing Lu, Hao Xu, Dongyue Wang, Yanlu Chen, Takeshi Inuoe, Liang Gao, Kai Lei

Figure extracted from Lu, *et al.* made available under a CC-BY 4.0 International license.

Rapid depletion and super-resolution microscopy reveal an unexpected role of the nuclear-speckle protein SRSF5 in paraspeckle assembly and dynamics during cellular stress
Benjamin Arnold, Laurell Kessler, Ellen Kazumi Okuda, Ricarda J. Riegger, Maria Clara Hernández Cañás, Ewelina Zebrowska, Cem Bakisoglu, Helder Y. Nagasse, David Stanek, Dorothee Dormann, Kathi Zarnack, Mike Heilemann, Michaela Müller-McNicoll

Spatial localization of CD16a at the human NK cell ADCC lytic synapse
Patrick Ross, Hijab Fatima, Dan P. Leaman, Jessica Matthias, Kathryn Spencer, Michael B. Zwick, Scott C. Henderson, Emily M. Mace, Charles Daniel Murin