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How to Minimise Photodamage with LED illumination

Sponsored by CoolLED, on 15 November 2023

Both fixed and live samples are susceptible to photodamage during fluorescence microscopy, which can shorten time-lapse experiments and cast doubt on observations. LED illumination for widefield fluorescence has the potential to counter this serious problem, whether running a complete experiment or simply finding the region of interest prior to confocal microscopy. Exposing biological samples to
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Analyzing calcium imaging data using Python

Posted by , on 27 October 2023

Andrey AndreevCalifornia Institute of Technology, David Prober labaandreev@caltech.edu Calcium imaging allows tracking neural activity in time with single-cell resolution. There are many questions you might be interested in answering using this technique: which cells are tuned to the stimuli? is there periodic activity present in the data? which cells act together? how complex is the activity
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behind DeXtrusion: an automated pipeline to detect epithelial cell extrusion through machine learning

Posted by , on 6 October 2023

The problem In animals, epithelial tissue covers most of our organs. In order to create the very intricate and complex shapes of these organs, these tissues are drastically remodelled during embryogenesis through growth and shape changes. Once they achieve these final shapes, they maintain their cell number in a dynamic equilibrium. Both of these mechanisms,
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How to… Publish Images blog series

Posted by , on 27 September 2023

by Helena Jambor In biology, images document appearances or structures of animals, issues, or cells, and increasingly are a source of quantitative insights of biological processes. Many papers are published every single day with images (Lee, 2018).Not all of these images are truthful or insightful. Some images are manipulated to mislead audiences (Bik et al.,
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The FocalPlane Network: joining and using the database

Posted by , on 20 September 2023

Our instructional videos take you step-by-step through the process of joining and using the FocalPlane Network. You can contact us at focalplane@biologists.com with any feedback on the Network. Joining the FocalPlane Network Using the FocalPlane Network
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An interview with Helen Spiers

Posted by , on 18 September 2023

Citizen science is a fantastic way to engage the public with scientific research by getting them involved with real research projects. For scientists, the power of the crowd can help them overcome analysis bottlenecks that might otherwise slow the pace of their research. Martin Jones and Lucy Collinson, from the Crick Electron Microscopy Science Technology
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All things unequal – live microscopy reveals asymmetric RNA localization in the early mammalian embryo

Posted by , on 4 September 2023

Authors: Jennifer Zenker1,*, Julian Heng2 1Australian Regenerative Medicine Institute (ARMI), Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Australia 2Remotely Consulting, Perth, Australia *Corresponding author Non-mammalian systems have been invaluable for us to decipher evolutionarily-conserved processes, orthologous molecules and cellular phenomena underlying early embryo development. One fundamental discovery from the work on non-mammalian
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Sharing Your Poster on Figshare: A Community Guide to How-To and Why

Posted by , on 26 July 2023

Join the Open Science revolution and increase the impact of your research by sharing it on Figshare. Discover the benefits of transparency, collaboration, and accessibility in science as you make your work citable, shareable, and discoverable. Our step-by-step guide will walk you through uploading your conference posters and data, ensuring your research reaches a wider
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Tracking in napari

Posted by , on 1 June 2023

In this blog post we are exploring cell tracking in napari. Two important processes in normal tissue development and disease are cell migration and proliferation. To gain a better understanding on these processes, tracking in time-lapse datasets is needed. By measuring track properties, like velocity and the total travelled distance, spatio-temporal relationships can be studied.
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Feature extraction in napari

Posted by , on 3 May 2023

This blog post revolves around extracting and selecting features from segmented images. We will define the terms feature extraction and selection. Also, we will learn how to categorize features and can look up specific features in a glossary. Furthermore, we will explore how to extract features in napari. Definition of feature extraction During feature extraction,
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