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Imaging nuclear dynamics and chromatin organization in a live intact organism: the design of the minimal constraint device

Posted by Dana Lorber, on 9 March 2022

To visualize the response of the nucleus and chromatin to mechanical signals, I developed a device that enables their imaging in a live intact Drosophila larva. Together with genetically encoded fluorescent markers, we can track muscle contraction and nuclear dynamics, as shown in Movie 1. While the muscle is at rest, we can image chromatin

If you can detect it, you can track it - recent TrackMate developments

Posted by Joanna Pylvänäinen, on 17 February 2022

TrackMate is your buddy for your everyday tracking TrackMate (Tinevez et al., 2017) is an open-source Fiji plugin initially made for tracking single particles imaged with fluorescence microscopes. It has now been updated to include a collection of new detectors and direct implementation of deep-learning models (Ershov, Phan, Pylvänäinen, Rigaud et al., 2021). In addition

Microgametogenesis in four dimensions live-cell imaging of this highly dynamic process in P. falciparum

Posted by FocalPlane, on 27 October 2021

Here is the preLight post from our first preLights – FocalPlane social writing event. 4D live-cell imaging of microgametogenesis in the human malaria parasite Plasmodium falciparum. Sabrina Yahiya, Sarah Jordan, Holly X. Smith, David C. A. Gaboriau, Mufuliat T. Famodimu, Farah A. Dahalan, Alisje Churchyard, George W. Ashdown, Jack Baum Preprint posted on July 29,

Phototoxicity - the good, the bad and the quantified.

Posted by Philippe Laissue, on 14 May 2021

Our virtual meeting on phototoxicity was held in late January 2021, generously sponsored by the European Microscopy Society and enabled by the Royal Microscopical Society. In four hours, spread over two days, the five organisers and twenty invited participants discussed the problem of phototoxicity in live imaging, and how we can start to tackle this

Imaging Specialist - University of Warwick, UK

Posted by Claire Mitchell, on 22 February 2021

Fixed Term Contract until 30 September 2022 The postholder, funded through a Wellcome Multi-user equipment award, will provide expert-level hands-on technical and user support for lattice light sheet microscopy. The appointee will need to combine enthusiasm and careful attention to detail with adaptability. The post would suit an individual with either a background in physics

Technology highlights - Quantitative Phase Imaging (QPI)

Posted by Johanna Bischof, on 20 January 2021

Interview with Helena Chmelová, Ph.D. from the Light Microscopy Core facility at the Institute of Molecular Genetics of the Czech Academy of Sciences in Prague, Czech Republic. Can you tell us a little bit about yourself – where you work, what your research focus is? I work at the Light Microscopy Core Facility at the Institute of

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Microscopy-related articles from our journals

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples Development 2024 151: dev202307
filoVision – using deep learning and tip markers to automate filopodia analysis J Cell Sci 2024 137: jcs261274
C omputational tools for quantifying actin filament numbers, lengths, and bundling Biology Open 2024 13: bio.060267

Microscopy-related preprint highlights

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