Microscopy preprints – applications in biology

Here is a curated selection of preprints published recently. In this post, we share preprints that use microscopy tools to answer questions in biology.

Centrosome positioning independently of microtubule-based forces
Alexandre Schaeffer, Morgan Gazzola, Matthieu Gelin, Benoit Vianay, Chiara de Pascalis, Laurent Blanchoin, Manuel Théry

Structural plasticity of bacterial ESCRT-III protein PspA in higher-order assemblies
Benedikt Junglas, Esther Hudina, Philipp Schönnenbeck, Ilona Ritter, Anja Heddier, Beatrix Santiago-Schübel, Pitter F. Huesgen, Dirk Schneider, Carsten Sachse

Structural basis for Vipp1 membrane binding: From loose coats and carpets to ring and rod assemblies
Benedikt Junglas, David Kartte, Mirka Kutzner, Nadja Hellmann, Ilona Ritter, Dirk Schneider, Carsten Sachse

Large transient assemblies of Apaf1 constitute the apoptosome in cells
Alicia C. Borgeaud, Iva Ganeva, Calvin Klein, Amandine Stooss, Daniela Ross-Kaschitza, Liyang Wu, Joel S. Riley, Stephen W.G. Tait, Thomas Lemmin, Thomas Kaufmann, Wanda Kukulski

Movement of the endoplasmic reticulum is driven by multiple classes of vesicles marked by Rab-GTPases
Allison Langley, Sarah Abeling-Wang, Erinn Wagner, John Salogiannis

Mechanisms of axoneme and centriole elimination in Naegleria gruberi
Alexander Woglar, Coralie Busso, Gabriela Garcia-Rodriguez, Friso Douma, Mary-Claude Croisier-Coeytaux, Graham Knott, Piere Gönczy

Compositionally unique mitochondria in filopodia support cellular migration
Madeleine Marlar-Pavey, Daniel Tapias-Gomez, Marcel Mettlen, Jonathan R Friedman

Cryo-electron tomography reveals the microtubule-bound form of inactive LRRK2
Siyu Chen, Tamar Basiashvili, Joshua Hutchings, Marta Sanz Murillo, Amalia Villagran Suarez, Jaime Alegrio Louro, Andres E. Leschziner, Elizabeth Villa

A disease resistance protein triggers oligomerization of its NLR helper into a hexameric resistosome to mediate innate immunity
Jogi Madhuprakash, AmirAli Toghani, Mauricio P. Contreras, Andres Posbeyikian, Jake Richardson, Jiorgos Kourelis, Tolga O. Bozkurt, Michael Webster, Sophien Kamoun

Using Photoswitching FRET to Define the Interaction Boundaries between the Rab1b GTPase and Secretory Cargo
Yehonathan Malis, Guy M. Hirschberg, George H. Patterson, Koret Hirschberg

Mechanosensitive FHL2 tunes endothelial function
Shailaja Seetharaman, John Devany, Ha Ram Kim, Emma van Bodegraven, Theresa Chmiel, Shentu Tzu-Pin, Wen-hung Chou, Yun Fang, Margaret Lise Gardel NC 4.0

Ccdc66 regulates primary cilium stability, disassembly and signaling important for epithelial organization
Jovana Deretic, Seyma Cengiz-Emek, Ece Seyrek, Elif Nur Firat-Karalar

Microtubule pivoting driven by spindle elongation rescues polar chromosomes to ensure faithful mitosis
Isabella Koprivec, Valentina Štimac, Petra Mikec, Iva M. Tolić

Mapping protein distribution in the canine photoreceptor sensory cilium and calyceal processes by ultrastructure expansion microscopy
Kei Takahashi, Raghavi Sudharsan, William A. Beltran

Chaperone-mediated heterotypic phase separation regulates liquid-to-solid phase transitions into amyloid fibrils
Sandeep K. Rai, Roopali Khanna, Anusha Sarbahi, Ashish Joshi, Samrat Mukhopadhyay

Life Cycle and Morphogenetic Differentiation in Heteromorphic Cell Types of a Cosmopolitan Marine Microalga
Laurie Bousquet, Shai Fainsod, Johan Decelle, Omer Murik, Fabien Chevalier, Benoit Gallet, Rachel Templin, Yannick Schwab, Yoav Avrahami, Gil Koplovitz, Chuan Ku, Miguel Frada

Nuclear RNA forms an interconnected network of transcription-dependent and tunable microgels
Mattia Marenda, Davide Michieletto, Rafal Czapiewski, Jon Stocks, Sophie M Winterbourne, Jamilla Miles, Olivia CA Fleming, Elena Lazarova, Michael Chiang, Graeme R Grimes, Hannes Becher, Atlanta Cook, Davide Marenduzzo, Ryu-Suke Nozawa, Nick Gilbert

Cell divisions imprint long lasting elastic strain fields in epithelial tissues
Ali Tahaei, Romina Pisticello-Gómez, S Suganthan, Greta Cwikla, Jana F. Fuhrmann, Natalie A. Dye, Marko Popović

Force-activated zyxin assemblies coordinate actin nucleation and crosslinking to orchestrate stress fiber repair
Donovan Y.Z. Phua, Xiaoyu Sun, Gregory M. Alushin

The WAVE complex forms linear arrays at negative membrane curvature to instruct lamellipodia formation
Muziyue Wu, Paul Marchando, Kirstin Meyer, Ziqi Tang, Derek N. Woolfson, Orion D. Weiner

Growth-induced physiological hypoxia correlates with growth deceleration during normal development
Yifan Zhao, Cyrille Alexandre, Gavin Kelly, Gantas Perez-Mockus, Jean-Paul Vincent

Transport and Organization of Individual Vimentin Filaments Within Dense Networks Revealed by Single Particle Tracking and 3D FIB-SEM
Bhuvanasundar Renganathan, Andrew Moore, Wei-Hong Yeo, Alyson Petruncio, David Ackerman, Aubrey Wiegel, The CellMap Team, H. Amalia Pasolli, C. Shan Xu, Gleb Shtengel, Harald F. Hess, Anna S Serpinskaya, Hao F. Zhang, Jennifer Lippincott-Schwartz, Vladimir I. Gelfand

Light-microscopy based dense connectomic reconstruction of mammalian brain tissue
Mojtaba R. Tavakoli, Julia Lyudchik, Michał Januszewski, Vitali Vistunou, Nathalie Agudelo, Jakob Vorlaufer, Christoph Sommer, Caroline Kreuzinger, Barbara Oliveira, Alban Cenameri, Gaia Novarino, Viren Jain, Johann Danzl

Dynamics of bacterial biofilm development imaged using light sheet fluorescence microscopy
Lenka Smerdova, Tibor Fuzik, Lucie Valentova, Pavol Bardy, Michaela Prochazkova, Martina Parenicova, Pavel Plevka

Under or Over? Tracing Complex DNA Topologies with High Resolution Atomic Force Microscopy
Max C. Gamill, Elizabeth P. Holmes, James I. Provan, Laura Wiggins, Renáta Rusková, Sylvia Whittle, Thomas E. Catley, Kavit H. S. Main, Neil Shephard, Helen. E. Bryant, Dušan Račko, Sean D. Colloms, Alice L. B. Pyne

Adhesive interactions within microbial consortia can be differentiated at the single-cell level through expansion microscopy
Pu-Ting Dong, Wenyuan Shi, Xuesong He, Gary G. Borisy

Long-term single molecule localization microscopy uncovers dynamic co-assembly of Lrp6 and Ror2 into Wnt-signalosomes
Michael Philippi, Julia Dohle, Isabelle Watrinet, Michael Holtmannspötter, Jinye Li, Oliver Birkholz, Yi Miao, Ulrich Rothbauer, K. Christopher Garcia, Rainer Kurre, Jacob Piehler, Changjiang You

Coherent Raman microscopy detects nucleolar defects through amide I peak shifts originating from β-sheets: an application to visualizing ongoing cellular senescence
Shigeo Ishibashi, Akihito Inoko, Yuki Oka, Philippe Leproux, Hideaki Kano

Clearing-enabled light sheet microscopy as a novel method for three-dimensional mapping of the sensory innervation of the mouse knee
Frank C. Ko, Spencer Fullam, Hoomin Lee, Shingo Ishihara, Natalie S. Adamczyk, Alia M. Obeidat, Sarah Soorya, Richard J. Miller, Anne-Marie Malfait, Rachel E. Miller

Spectrally Resolved Single Molecule Orientation Imaging Reveals Direct Correspondence between Polarity and Order Experienced by Nile Red in Supported Lipid Bilayer Membrane
Aranyak Sarkar, Jyotsna Bhatt Mitra, Veerendra K. Sharma, Vinu Namboodiri, Manoj Kumbhakar

Diverse effects of fluorescent labels on alpha-synuclein condensate formation during liquid-liquid phase separation
Mantas Ziaunys, Darius Sulskis, Dominykas Veiveris, Andrius Sakalauskas, Kamile Mikalauskaite, Vytautas Smirnovas

A look beyond topography: transient phenomena of Escherichia coli cell division captured with high-speed in-line force mapping
Christian Ganser, Shigetaka Nishiguchi, Feng Yueh Chan, Takayuki Uchihashi

Cryo-electron tomography pipeline for plasma membranes
Willy W. Sun, Dennis J. Michalak, Kem A. Sochacki, Prasanthi Kunamaneni, Marco A. Alfonzo-Méndez, Andreas M. Arnold, Marie-Paule Strub, Jenny E. Hinshaw, Justin W. Taraska

Mechanical imbalance between normal and transformed cells drives epithelial homeostasis through cell competition
Praver Gupta, Sayantani Kayal, Nobuyuki Tanimura, Shilpa P. Pothapragada, Harish K. Senapati, Padmashree Devendran, Yasuyuki Fujita, Dapeng Bi, Tamal Das

Tks5 interactome reveals ER-associated machinery translation in invadosomes
Léa Normand, Benjamin Bonnard, Margaux Sala, Sylvaine Di-Tommaso, Cyril Dourthe, Anne-Aurélie Raymond, Jean-William Dupuy, Luc Mercier, Jacky G. Goetz, Violaine Moreau, Elodie Henriet, Frédéric Saltel

Intravital imaging reveals glucose-dependent cilia movement in pancreatic islets in vivo
Olha Melnyk, Jeff Kaihao Guo, Zipeng Alex Li, Jing W. Hughes, Amelia K. Linnemann

Clathrin-associated carriers enable recycling through a kiss-and-run mechanism
Jiachao Xu, Yu Liang, Nan Li, Song Dang, Amin Jiang, Yiqun Liu, Yuting Guo, Xiaoyu Yang, Yi Yuan, Xinyi Zhang, Yaran Yang, Yongtao Du, Anbing Shi, Xiaoyun Liu, Dong Li, Kangmin He

The actomyosin system is essential for the integrity of the endosomal system in bloodstream form Trypanosoma brucei
Fabian Link, Sisco Jung, Xenia Malzer, Felix Zierhut, Antonia Konle, Alyssa Borges, Christopher Batters, Monika Weiland, Mara Pöllmann, An Binh Nguyen, Johannes Kullmann, Claudia Veigel, Markus Engstler, Brooke Morriswood

Real-time Biomechanical Characterisation of Cytoskeletal Remodelling
Kaiwen Zhang, Chayla Reeves, Joseph D. Berry, Kate Fox, Aaron Elbourne, Amy Gelmi

Hotwiring integrin endocytosis acutely modulates cell interactions
Sahil Kamboj, Alphonse Boché, Anneline Moret, Zixu Wang, Carole Aimé, Rémy Agniel, Johanne Leroy-Dudal, Franck Carreiras, Olivier Gallet, Stephen J Royle, Ambroise Lambert

Compositionally unique mitochondria in filopodia support cellular migration
Madeleine Marlar-Pavey, Daniel Tapias-Gomez, Marcel Mettlen, Jonathan R Friedman

PDZD8-FKBP8 tethering complex at ER-mitochondria contact sites regulates mitochondrial complexity
Koki Nakamura, Saeko Aoyama-Ishiwatari, Takahiro Nagao, Mohammadreza Paaran, Christopher J. Obara, Yui Sakurai-Saito, Jake Johnston, Yudan Du, Shogo Suga, Masafumi Tsuboi, Makoto Nakakido, Kouhei Tsumoto, Yusuke Kishi, Yukiko Gotoh, Chulhwan Kwak, Hyun-Woo Rhee, Jeong Kon Seo, Hidetaka Kosako, Clint Potter, Bridget Carragher, Jennifer Lippincott-Schwartz, Franck Polleux, Yusuke Hirabayashi

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