Microscopy preprints – applications in biology

Posted by FocalPlane, on 21 February 2025

Here is a curated selection of preprints published recently. In this post, we share preprints that use microscopy tools to answer questions in biology.

High-Resolution Structures of Tobacco Mosaic Virus Disks from Cryo-Electron Microscopy
Ismael Abu-Baker, Artur P. Biela, Sachin N. Shah, Jonathan G. Heddle, George P. Lomonossoff, Amy Szuchmacher Blum

Memory engram synapse 3D molecular architecture visualized by cryoCLEM-guided cryoET
Charlie Lovatt, Thomas J. O’Sullivan, Clara Ortega-de San Luis, Tomás J. Ryan, René A. W. Frank

Atomic Force Microscopy reveals differences in mechanical properties linked to cortical structure in mouse and human oocytes
Rose Bulteau, Lucie Barbier, Guillaume Lamour, Yassir Lemseffer, Marie-Hélène Verlhac, Nicolas Tessandier, Elsa Labrune, Martin Lenz, Marie-Emilie Terret, Clément Campillo

Figure extracted from Bulteau, *et al.* The image is made available under a CC-BY 4.0 International license.

Fibroblast contractility drives network reorganization and epithelial proliferation in intestinal polyposis
Mei-Lan Li, Yuying Wang, Maria Figetakis, David Gonzalez, Jason Jin, Elizabeth S. McDonald, Nadia A. Ameen, Kaelyn Sumigray

Mitochondria transported by Kinesin 3 prevent localized calcium spiking to inhibit caspase-dependent specialized cell death
Rashna Sharmin, Aladin Elkhalil, Sara Pena, Pranya Gaddipati, Ginger Clark, Pavak K. Shah, Mark W. Pellegrino, Shai Shaham, Piya Ghose

Image-based screens identify regulators of endogenous Dvl2 biomolecular condensates
Antonia Schubert, Florian Heigwer, Christian Scheeder, Oksana Voloshanenko, Dominique Kranz, Franziska Ragaller, Nadine Winkler, Thilo Miersch, Barbara Schmitt, Melanie Kuhse, Daniel Gimenes, Diana Ordoñez-Rueda, Jennifer Schwarz, Frank Stein, Dirk Jäger, Ulrike Engel, Michael Boutros

Fluorescence lifetime imaging microscopy for metabolic analysis of LDHB inhibition in triple negative breast cancer
A. Galloway, B. Ter Hofstede, Alex J. Walsh

Timelapse and volumetric imaging of mitochondrial networking using NAD(P)H autofluorescence via 2-photon microscopy
Blanche ter Hofstede, Alex J. Walsh

Quantifying the organization and dynamics of M. smegmatis morphology from Long-Term Time-Lapse Atomic Force Microscopy
Clément Soubrier, Anotida Madzvamuse, Haig Alexander Eskandarian, Khanh Dao Duc

Figure extracted from Soubrier, *et al.* The image is made available under a CC-BY 4.0 International license.

Quantitative Spatial Analysis of Chromatin Biomolecular Condensates using Cryo-Electron Tomography
Huabin Zhou, Joshua Hutchings, Momoko Shiozaki, Xiaowei Zhao, Lynda K. Doolittle, Shixin Yang, Rui Yan, Nikki Jean, Margot Riggi, Zhiheng Yu, Elizabeth Villa, Michael K. Rosen

Cryogenic electron tomography and elemental analysis of mitochondrial granules in human retinal ganglion cells
Gong-Her Wu, Cathy Hou, Andrew Thron, Hirenkumar Rajendra Patel, Liam Spillane, Sanket Rajan Gupte, Serena Yeung-Levy, Sahil Gulati, Christopher Booth, Yaping Joyce Liao, Wah Chiu

Real-Time Analysis of Nanoscale Dynamics in Membrane Protein Insertion via Single-Molecule Imaging
C. Yang, D. Ma, S. Hu, M. Li, Y. Lu

Fluorescence Lifetime Imaging Microscopy (FLIM) visualizes internalization and biological impact of nanoplastics in live intestinal organoids
Irina A. Okkelman, Hang Zhou, Sergey M. Borisov, Angela C. Debruyne, Austin E. Y. T. Lefebvre, Marcelo Leomil Zoccoler, Linglong Chen, Bert Devriendt, Ruslan I. Dmitriev

Insect wings arose with a genetic circuit that extends the useful range of a BMP morphogen
Anqi Huang, Luca Cocconi, Ben Nicholls-Mindlin, Cyrille Alexandre, Guillaume Salbreux, Jean-Paul Vincent

Ångström-resolution imaging of cell-surface glycans
Luciano A. Masullo, Karim Almahayni, Isabelle Pachmayr, Monique Honsa, Larissa Heinze, Sarah Fritsche, Heinrich Grabmayr, Ralf Jungmann, Leonhard Möckl

Polarity reversal of stable microtubules during neuronal development
Malina K. Iwanski, Albert K. Serweta, H. Noor Verwei, Bronte C. Donders, Lukas C. Kapitein

Cryo-correlative light and electron tomography of dopaminergic axonal varicosities reveals non-synaptic modulation of cortico-striatal synapses
Paul Lapios, Robin Anger, Vincent Paget-Blanc, Esther Marza, Vladan Lučić, Rémi Fronzes, Etienne Herzog, David Perrais

Cell heterogeneity and fate bistability drive tissue patterning during intestinal regeneration
C. Schwayer, S. Barbiero, D. B. Brückner, C. Baader, N. A. Repina, O. E. Diaz, L. Challet Meylan, V. Kalck, S. Suppinger, Q. Yang, J. Schnabl, U. Kilik, J. G. Camp, B. Stockinger, M. Bühler, M. B. Stadler, E. Hannezo, P. Liberali

Structural and functional characterization of integrin α5-targeting antibodies for anti-angiogenic therapy
Adam Nguyen, Joel B. Heim, Gabriele Cordara, Matthew C. Chan, Hedda Johannesen, Cristine Charlesworth, Ming Li, Caleigh M. Azumaya, Benjamin Madden, Ute Krengel, Alexander Meves, Melody G. Campbell

Figure extracted from Nguyen, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

Distinct filament morphology and membrane tethering features of the dual FtsZs in Odinarchaeota
Jayanti Kumari, Akhilesh Uthaman, Ananya Kundu, Anubhav Dhar, Vaibhav Sharma, Sucharita Bose, Soumyajit Dutta, Srijita Roy, Ramanujam Srinivasan, Samay Pande, Kutti R. Vinothkumar, Pananghat Gayathri, Saravanan Palani

Geometry-driven asymmetric cell divisions pattern cell cycles and zygotic genome activation in the zebrafish embryo
Nikhil Mishra, Yuting I. Li, Edouard Hannezo, Carl-Philipp Heisenberg

Lipid packing and local geometry influence septin curvature sensing
Brandy N. Curtis, Ellysa J. D. Vogt, Christopher Edelmaier, Amy S. Gladfelter

Spatiotemporal temperature control by holographic heating microscopy unveils cellular thermosensitive calcium signaling
Kotaro Oyama, Ayumi Ishii, Shuhei Matsumura, Tomoko Gowa Oyama, Mitsumasa Taguchi, Madoka Suzuki

Population-level morphological analysis of paired CO2- and odor-sensing olfactory neurons in D. melanogaster via volume electron microscopy
Jonathan Choy, Shadi Charara, Kalyani Cauwenberghs, Quintyn McKaughan, Keun-Young Kim, Mark H. Ellisman, Chih-Ying Su

Figure extracted from Choy, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

SRS microscopy identifies inhibition of vitellogenesis as a mediator of lifespan extension by caloric restriction in C. elegans
Bowen Yang, Bryce Manifold, Wuji Han, Catherin DeSousa, Wanyi Zhu, Aaron Streets, Denis V. Titov

Figure extracted from Yang, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

Microscopy-Guided Spatial Proteomics Reveals Novel Proteins at the Mitochondria-Lipid Droplet Interface and Their Role in Lipid Metabolism
Yen-Ming Lin, Weng Man Chong, Chun-Kai Huang, Hsiao-Jen Chang, Chantal Hoi Yin Cheung, Jung-Chi Liao

Actomyosin and the Arp2/3 Complex Are Involved in the Internalization of Cellulose Synthase Complexes
Liyuan Xu, Weiwei Zhang, Lei Huang, Chunhua Zhang, Christopher J. Staiger

Figure extracted from Xu, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

Super-resolution compatible DNA labeling technique reveals chromatin mobility and organization changes during differentiation
Maruthi K. Pabba, Miroslav Kuba, Tomáš Kraus, Kerem Celikay, Janis Meyer, Sunik Kumar Pradhan, Andreas Maiser, Hartmann Harz, Heinrich Leonhardt, Karl Rohr, Michal Hocek, M. Cristina Cardoso

Figure extracted from Pabba, *et al.* The image is made available under a CC-BY-NC 4.0 International license.

Super-resolution microscopy of mitochondrial mRNAs
Stefan Stoldt, Frederike Maass, Michael Weber, Sven Dennerlein, Peter Ilgen, Jutta Gärtner, Aysenur Canfes, Sarah V. Schweighofer, Daniel C. Jans, Peter Rehling, Stefan Jakobs

Ultrastructure expansion microscopy of axonemal dynein in islet primary cilia
Xinhang Dong, Jeong Hun Jo, Jing Hughes

Mapping Alzheimer Disease Molecular Pathologies in Large-Scale Connectomics Data: A Publicly Accessible Correlative Microscopy Resource
Xiaomeng Han, Peter H. Li, Shuohong Wang, Tim Blakely, Sneha Aggarwal, Bhavika Gopalani, Morgan Sanchez, Richard Schalek, Yaron Meirovitch, Zudi Lin, Daniel Berger, Yuelong Wu, Fatima Aly, Sylvie Bay, Benoît Delatour, Pierre Lafaye, Hanspeter Pfister, Donglai Wei, Viren Jain, Hidde Ploegh, Jeff Lichtman

Scanning Electron Microscopy Study of Bacterial Growth in Mycelial Extracellular Matrices
Davin Browner, Andrew Adamatzky

Figure extracted from Browner and Adamatzky. The image is made available under a CC-BY-NC 4.0 International license.

GLP-1R associates with VAPB and SPHKAP at ERMCSs to regulate β-cell mitochondrial remodelling and function
Gregory Austin, Affiong I. Oqua, Liliane El Eid, Mingli Zhu, Yusman Manchanda, Priyanka Peres, Helena Coyle, Yelyzaveta Poliakova, Karim Bouzakri, Alex Montoya, Dominic J. Withers, Michele Solimena, Ben Jones, Steven J. Millership, Steffen Burgold, David C.A. Gaboriau, Endre Majorovits, Inga Prokopenko, Jonathon Nixon-Abell, Andreas Müller, Alejandra Tomas

Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons
Jana Kroll, Uljana Kravčenko, Mohsen Sadeghi, Christoph A. Diebolder, Lia Ivanov, Małgorzata Lubas, Thiemo Sprink, Magdalena Schacherl, Mikhail Kudryashev, Christian Rosenmund