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SRRF-Stream+ Super-Resolution Microscopy Accessible to All

Sponsored by Andor, on 12 August 2020

Fast, reliable & live-cell compatible Super-Resolution Science has limits imposed by the laws of physics that constrain discoveries and the advance of knowledge. In microscopy, up until the beginning of the XXI century, the diffraction limit of light was an unbreakable barrier. This law of physics imposes that two points could not be resolved (clearly
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Science and Art – the not so odd couple?

Posted by , on 31 July 2020

At a first, superficial glance, you could be forgiven for placing scientists and artists at the opposite ends of the career spectrum. Scientists need to be accurate and methodical. They must generate highly reproducible data while adhering to strict regulations. On the other hand, artists are often stereotyped as disorganized, free spirits, ungoverned by rules,
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“openFrame” for modular, extensible, easily maintained, open-source microscopy

Posted by , on 23 July 2020

The openFrame [1] is an open-source microscopy hardware project initiated by the Photonics Group in the Physics Department at Imperial College London that is intended to provide access to a modular, upgradable, easily maintained and modifiable microscope frame that can be implemented at relatively low cost compared to existing commercial instruments. openFrame is a component
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Electron microscopy: from the dark ages to a bright future

Posted by , on 21 July 2020

Good sample preparation is, as every microscopist knows, the key to delivering sound results from an imaging experiment. In the digital age, and with the advent of big data, image analysis is also critical to extract meaningful quantitative results from image data. Indeed nowadays, the microscope itself is often the most well-developed and user-friendly part
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Etch A Cell - segmenting electron microscopy data with the power of the crowd

Posted by , on 16 July 2020

Recent years have seen remarkable developments in imaging techniques and technologies, producing increasingly rich datasets that require huge amounts of costly technological infrastructure, computational power and researcher effort to process. Techniques such as light-sheet microscopy and volume electron microscopy routinely generate terabytes worth of data overnight. With a single data acquisition producing more images than
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Primers on Microscopy for Biologists - Resolution

Posted by , on 15 July 2020

Formal definitions of resolution refer to imaginary objects such as infinitely small sources of light. I will avoid those and instead try to provide a pragmatic explanation. Practically, the spatial resolution is the size of the smallest structure that can be distinguished in the light coming from a specimen. All sensors and components of the
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Microscope technology development and the search for a free lunch

Posted by , on 10 July 2020

I fortuitously stumbled into the field of microscopy around 2013, just in time to enjoy the hype of the 2014 Chemistry Nobel Prize for super-resolved fluorescence imaging. As a newcomer, I was quite surprised that this nearly 400-year-old field could still catch the attention of Stockholm. After all, wasn’t a microscope just some lenses to
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My history with GFP (and other members of the family)

Posted by , on 7 July 2020

I first crossed paths with green fluorescent protein (GFP) while doing an internship in 1996 at the Max Planck Institute in Göttingen. I was chemically labeling epidermal growth factor (EGF) with the fluorescent dyes Cy3 and Cy5 to study growth-factor signaling. Two post-docs were discussing the use of GFP as an alternative fluorescent tag and
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Open microscopy across our journals

Posted by , on 3 July 2020

21-27 October marked International Open Access Week 2019. During this week many events were organised, stimulating discussion on open knowledge and connecting like-minded people and organisations that are all working towards greater accessibility of research. But of course important work in this area is being done all year round and we find ourselves in an
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Career insight: bioimage analysis

Posted by , on 3 July 2020

I am a postdoctoral fellow based at the Monash Institute of Pharmaceutical Sciences (MIPS), Melbourne, Australia. I am originally from Kerala, India and travelled to Australia for my postgraduate studies almost 13 years ago. I came to Melbourne because of all the high quality and affordable educational opportunities, and fell in love with it for
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