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Etch A Cell - segmenting electron microscopy data with the power of the crowd

Posted by Martin Jones, on 16 July 2020

Recent years have seen remarkable developments in imaging techniques and technologies, producing increasingly rich datasets that require huge amounts of costly technological infrastructure, computational power and researcher effort to process. Techniques such as light-sheet microscopy and volume electron microscopy routinely generate terabytes worth of data overnight. With a single data acquisition producing more images than

Primers on Microscopy for Biologists - Resolution

Posted by Nachiket Vartak, on 15 July 2020

Formal definitions of resolution refer to imaginary objects such as infinitely small sources of light. I will avoid those and instead try to provide a pragmatic explanation. Practically, the spatial resolution is the size of the smallest structure that can be distinguished in the light coming from a specimen. All sensors and components of the

Microscope technology development and the search for a free lunch

Posted by Alfred Millett-Sikking, on 10 July 2020

I fortuitously stumbled into the field of microscopy around 2013, just in time to enjoy the hype of the 2014 Chemistry Nobel Prize for super-resolved fluorescence imaging. As a newcomer, I was quite surprised that this nearly 400-year-old field could still catch the attention of Stockholm. After all, wasn’t a microscope just some lenses to

My history with GFP (and other members of the family)

Posted by Joachim Goedhart, on 7 July 2020

I first crossed paths with green fluorescent protein (GFP) while doing an internship in 1996 at the Max Planck Institute in Göttingen. I was chemically labeling epidermal growth factor (EGF) with the fluorescent dyes Cy3 and Cy5 to study growth-factor signaling. Two post-docs were discussing the use of GFP as an alternative fluorescent tag and

Open microscopy across our journals

Posted by FocalPlane, on 3 July 2020

21-27 October marked International Open Access Week 2019. During this week many events were organised, stimulating discussion on open knowledge and connecting like-minded people and organisations that are all working towards greater accessibility of research. But of course important work in this area is being done all year round and we find ourselves in an

Career insight: bioimage analysis

Posted by Pradeep Rajasekhar, on 3 July 2020

I am a postdoctoral fellow based at the Monash Institute of Pharmaceutical Sciences (MIPS), Melbourne, Australia. I am originally from Kerala, India and travelled to Australia for my postgraduate studies almost 13 years ago. I came to Melbourne because of all the high quality and affordable educational opportunities, and fell in love with it for

A welcome message from the Community Manager

Posted by Christos Kyprianou, on 1 July 2020

Hello everyone and welcome to FocalPlane! My name is Christos and I joined the Journal of Cell Science team at The Company of Biologists as the FocalPlane Community Manager about 9 months ago to help build and manage a community website designed to bring experts and non-experts in microscopy together under one roof. My background,

Origin story: FocalPlane

Posted by Sharon Ahmad, on 1 July 2020

On a sunny, summer’s day in July 2018, The Company of Biologists was hosting its annual lunch before a meeting with the Board of Directors and Editors-in-Chief. It’s a very friendly affair, and a great opportunity for the in-house staff to mingle with the Board and Editors – people they don’t see on a day-to-day

A 'lockdown post' from facility managers across the world

Posted by FocalPlane, on 1 July 2020

The year 2020 has been challenging so far. The current lockdown measures everywhere across the world brought about a change in how we work. But we have adapted and we have overcome this challenge in this new era of ‘working from home’. Here’s how people from imaging facilities in different parts of the world are

Advancing knowledge in mitochondria division using Dragonfly ultra-fast imaging

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Microscopy-related articles from our journals

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples Development 2024 151: dev202307
filoVision – using deep learning and tip markers to automate filopodia analysis J Cell Sci 2024 137: jcs261274
C omputational tools for quantifying actin filament numbers, lengths, and bundling Biology Open 2024 13: bio.060267

Microscopy-related preprint highlights

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