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The Third Euro-BioImaging User Forum: “At the Forefront of Neuroscience”

Posted by , on 17 March 2022

Euro-BioImaging is organizing a third online User Forum on Tuesday, April 5, 2022 from 14:00-17:00 CEST. The topic is “At the Forefront of Neuroscience.” This event will highlight the importance of cutting-edge imaging technologies in support of brain research and showcase the specific expertise available at our Nodes across Europe through case studies presented in tandem with the research community.  Program:
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Imaging nuclear dynamics and chromatin organization in a live intact organism: the design of the minimal constraint device

Posted by , on 9 March 2022

To visualize the response of the nucleus and chromatin to mechanical signals, I developed a device that enables their imaging in a live intact Drosophila larva.   Together with genetically encoded fluorescent markers, we can track muscle contraction and nuclear dynamics, as shown in Movie 1.  While the muscle is at rest, we can image chromatin
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If you can detect it, you can track it - recent TrackMate developments

Posted by , on 17 February 2022

TrackMate is your buddy for your everyday tracking  TrackMate (Tinevez et al., 2017) is an open-source Fiji plugin initially made for tracking single particles imaged with fluorescence microscopes. It has now been updated to include a collection of new detectors and direct implementation of deep-learning models (Ershov, Phan, Pylvänäinen, Rigaud et al., 2021). In addition
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Revealing the spatial regulation of the transcriptome using Split-FISH

Sponsored by Andor, on 9 February 2022

Featured Lab: The Chen Lab, Genome Institute of Singapore  As cells develop and function in tissues, many changes occur not just at the level of RNA transcription, but also at the larger level of spatial organisation of cellular gene expression within the tissue. Studying these changes to RNA profiles at the single-cell level can thus provide
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DeepImageJ: Deep learning in bioimage analysis for dummies

Posted by , on 2 December 2021

Written by Estibaliz Gómez-de-Mariscal, Cristina de-la-Torre-Gutiérrez, Arrate Muñoz-Barrutia, and Daniel Sage Do you invest significant time looking for tools to analyze your microscopy images? Probably you have already heard about the extraordinary capabilities of deep learning (DL) to reveal complex visual structures in images. If you are a biologist practicing daily ImageJ, you may look
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Overview of Microscopy Techniques: Confocal, Widefield, Transmitted Light and Deconvolution

Sponsored by Andor, on 30 November 2021

Microscopes are an essential tool for modern life science applications. From medical diagnostics (for example, identifying the source of an infection, or the severity of a tumour) to food analysis.  With such demand it is not surprising that in the last decade, there has been a boom in microscopy technologies allowing researchers to see beyond
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Primed conversion: the Basics

Posted by , on 15 November 2021

It has been a bit more than 6 years since the first “primed conversion” article1 was published in May 2015 and around 3 years since I first heard about this new photoconversion technique. I recall that it was almost 1 year after I got my MSc in Greece when I contacted Dr Periklis (Laki) Pantazis
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Meet BC43, Andor’s New Benchtop Confocal Microscope

Sponsored by Andor, on 8 November 2021

Andor Technology, an Oxford Instruments company and world leader in scientific imaging solutions, today announced the addition of BC43, a revolutionary benchtop confocal system, to its award-winning microscopy portfolio.  A Big Deal in a Small Box An affordable and high-speed confocal microscope, with a small footprint, BC43 is purposefully easy to use, allowing researchers to
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PUMA Open Source Multimodality 3D Printed Microscope

Posted by , on 26 October 2021

PUMA is an advanced direct vision 3D printed multimodality microscopy system with fluorescence ,phase contrast, Köhler illumination and augmented reality functions - and more.
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Temperature control in light microscopy – challenges and solutions

Sponsored by Interherence, on 9 September 2021

We describe common challenges that researchers face when trying to precisely control the temperature of the sample they are imaging. We aim to show that thermal control in high sensitivity microscopy is not trivial if you care about temperature directly in the field of view of the microscope (which you should). We hope to persuade
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