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Part V The future: The hope of smart microscopes and phantoms

Posted by Elisabeth Kugler, on 24 April 2021

Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact: kugler.elisabeth@gmail.com; emmanuel.reynaud@ucd.ie We are reaching the end of our LSFM journey. Now it is time to look into the future, riding the plane of light! So, what is next for the LSFM? 1. Four-to-two, then four, then one. The story of SPIM, and LSFM per se,

Technology highlights - Quantitative Phase Imaging (QPI)

Posted by Johanna Bischof, on 20 January 2021

Interview with Helena Chmelová, Ph.D. from the Light Microscopy Core facility at the Institute of Molecular Genetics of the Czech Academy of Sciences in Prague, Czech Republic. Can you tell us a little bit about yourself – where you work, what your research focus is? I work at the Light Microscopy Core Facility at the Institute of

Technology highlights - Traction Force Microscopy (TFM)

Posted by Johanna Bischof, on 9 December 2020

Interview with Aki Stubb, Ph.D. Please tell us a bit about yourself and the facility where you work. My name is Aki Stubb and I am a post-doctoral fellow at the University of Cambridge, UK. I did my PhD in the group of Johanna Ivaska at the Turku Bioscience Centre, University of Turku and Åbo

SRRF-Stream+ Super-Resolution Microscopy Accessible to All

Expansion microscopy

Posted by Shoh Asano, on 29 July 2020

Written by Shoh Asano and Ruixuan Gao Light microscopy and diffraction limit For centuries, light microscopy has played a central role in biological studies. The first implementations of a light microscope dates back to as early as the late 16th and early 17th century, when an array of polished lenses was used to magnify (biological)

“openFrame” for modular, extensible, easily maintained, open-source microscopy

Posted by Sunil Kumar, on 23 July 2020

The openFrame [1] is an open-source microscopy hardware project initiated by the Photonics Group in the Physics Department at Imperial College London that is intended to provide access to a modular, upgradable, easily maintained and modifiable microscope frame that can be implemented at relatively low cost compared to existing commercial instruments. openFrame is a component

How CLIJ2 can make your bio-image analysis workflows incredibly fast

Posted by Robert Haase, on 14 July 2020

Do you also spend a substantial amount of your time waiting for automated image analysis to finish? I did, again and again for more than a decade. And, then, the morning after running a script overnight, you realize that a parameter was wrongly set. It was about time to change that. History Processing on graphics

Lessons learned from an open-hardware project in microscopy: The mesoSPIM initiative

Posted by Fabian Voigt, on 1 July 2020

The mesoscale selective plane illumination microscope (mesoSPIM) is a highly versatile open-source light-sheet microscope for imaging large (cm-sized) cleared tissue samples. Using the documentation and software available via mesospim.org and on Github, interested labs can set up their own microscope. Currently, ten mesoSPIM instruments are in operation around the globe and several more are under

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Microscopy-related articles from our journals

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples Development 2024 151: dev202307
filoVision – using deep learning and tip markers to automate filopodia analysis J Cell Sci 2024 137: jcs261274
C omputational tools for quantifying actin filament numbers, lengths, and bundling Biology Open 2024 13: bio.060267

Microscopy-related preprint highlights

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