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Featured image with Ioakeim (Makis) Ampartzidis

Posted by FocalPlane, on 27 October 2023

Our featured image, ‘Dandelion in spring’ from Ioakeim (Makis) Ampartzidis, shows neuroepithelial-like cells cultured on glass beads. The cell nuclei are coloured in magenta, and the cytoskeleton is stained in black, labelling F-actin. The actively proliferating cells are highlighted with Ki-67 antigen in green. We caught up with Makis to find out more about his

Featured image with Victoria Alonso

Posted by FocalPlane, on 13 October 2023

Our featured image is a Trypanosoma cruzi epimastigote, prepared for expansion confocal microscopy with pan-proteome staining with NHS-ester Atto564 by Victoria Alonso. The image is a z-stack acquired with a Zeiss LSM 880 and was edited with ImageJ Fiji, plugin: Z-stack depth Colorcode.

Featured image with Peggy Paschke

Posted by FocalPlane, on 29 September 2023

Our featured image is called Dictyostelium Trees on Fire and was acquired by Peggy Paschke. The image shows Dictyostelium discoideum cells stably expressing the cAMP sensor Flamindo2. Cells were imaged as 5×5 tile scan for 5 hours with a Nikon AXR (488 nm excitation) using a 4x air objective. Shown is a cropped part of

Featured image with Martín Estermann

Posted by FocalPlane, on 15 September 2023

Our featured image, acquired by Martín Estermann, is a 3D reconstruction of C. elegans transgenic embryos. Embryos were imaged live using a Zeiss Celldiscoverer 7 microscope. The 3D reconstruction was performed using Fiji. Histones in are shown in green and the cell membrane in magenta. We caught up with Martín to find out about his

Featured image with Beste Senem Değirmenci

Posted by FocalPlane, on 18 August 2023

Our featured image, ‘Bubbles of The Cell’, shows enlarged endosomal vesicles in HeLa S3 cells upon inhibition of PI(3,5)P2 activity. PI(3,5)P2 is a crucial phosphoinositide involved in the regulation of endosomal trafficking. Depletion of PI(3,5)P2 disrupts the transition from early to late endosomes, leading to the enlargement of endosomal vesicles. Imaged with Leica DMI8 SP8

Featured image with Outi Paloheimo

Posted by FocalPlane, on 4 August 2023

Our featured image is laser scanning confocal microscope image showing cell division of Madin-Darby canine kidney cells with nuclei in grey and filamentous actin in orange. The image was acquired with a Zeiss LSM 780 microscope with a 63x/1.40 oil immersion objective, 405 nm diode laser and 561 nm diode-pumped solid-state laser. Deconvolution was performed

Featured image with David McGrath

Posted by FocalPlane, on 21 July 2023

Our featured image is of mature mouse brown adipocytes (WT-1 cell line) under normal conditions from David McGrath. Channel 1 (green LUT) shows lipid droplets stained with BODIPY 493/503 and channel 2 (fire LUT) shows mitochondria stained with TMRE. The image was obtained using a Visitron SD-TIRF confocal microscope (60x Apo TIRF (corr.) Oil/ NA: 1.49) at the UKE Microscopy Imaging Facility

Featured image with Ramiro Tomasina

Posted by FocalPlane, on 12 July 2023

Our featured image shows an ultrastructure expansion microscopy image of a procyclic Trypanosoma brucei labelled with anti-acetylated tubulin in magenta, DAPI in cyan and a flagellar marker in yellow. The image is a z-projection of maximum intensity. The image was acquired in the Trypanosoma cell biology unit at the Institut Pasteur of Paris (headed by Philippe Bastin),

Featured image with Simon Desiderio

Posted by FocalPlane, on 21 April 2023

Our featured image shows the developing dorsal root ganglia of an E12.5 mouse embryo from Simon Desiderio.

Featured image with Ellen Skarpen and José Teles Reis

Posted by FocalPlane, on 5 April 2023

Our featured image shows a Drosophila third instar larval brain with activated ‘Mosaic analysis with a repressible cell marker’ (MARCM) specifically in the Optic lobe, marked by GFP. This genetic technique allows for the labelling and manipulation of mitotically active clones of cells for the study of fate mapping or gene role through gain/loss of function. In this

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Microscopy-related articles from our journals

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples Development 2024 151: dev202307
filoVision – using deep learning and tip markers to automate filopodia analysis J Cell Sci 2024 137: jcs261274
C omputational tools for quantifying actin filament numbers, lengths, and bundling Biology Open 2024 13: bio.060267

Microscopy-related preprint highlights

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