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Electron microscopy: from the dark ages to a bright future

Posted by Lucy Collinson, on 21 July 2020

Good sample preparation is, as every microscopist knows, the key to delivering sound results from an imaging experiment. In the digital age, and with the advent of big data, image analysis is also critical to extract meaningful quantitative results from image data. Indeed nowadays, the microscope itself is often the most well-developed and user-friendly part

Take this quick X-Ray Microscopy Usage Survey!

Posted by Georgina Fletcher, on 17 July 2020

Take part in online poll to gauge use of technique and identify opportunities to expand its application The use of X-ray microscopy techniques is well established in many scientific and engineering disciplines but not all have “seen the light”. The X-ray Microscopy Focussed Interest Group (X-ray FIG) of the Royal Microscopical Society (RMS) would like

Etch A Cell - segmenting electron microscopy data with the power of the crowd

Posted by Martin Jones, on 16 July 2020

Recent years have seen remarkable developments in imaging techniques and technologies, producing increasingly rich datasets that require huge amounts of costly technological infrastructure, computational power and researcher effort to process. Techniques such as light-sheet microscopy and volume electron microscopy routinely generate terabytes worth of data overnight. With a single data acquisition producing more images than

Primers on Microscopy for Biologists - Resolution

Posted by Nachiket Vartak, on 15 July 2020

Formal definitions of resolution refer to imaginary objects such as infinitely small sources of light. I will avoid those and instead try to provide a pragmatic explanation. Practically, the spatial resolution is the size of the smallest structure that can be distinguished in the light coming from a specimen. All sensors and components of the

How CLIJ2 can make your bio-image analysis workflows incredibly fast

Posted by Robert Haase, on 14 July 2020

Do you also spend a substantial amount of your time waiting for automated image analysis to finish? I did, again and again for more than a decade. And, then, the morning after running a script overnight, you realize that a parameter was wrongly set. It was about time to change that. History Processing on graphics

Microscope technology development and the search for a free lunch

Posted by Alfred Millett-Sikking, on 10 July 2020

I fortuitously stumbled into the field of microscopy around 2013, just in time to enjoy the hype of the 2014 Chemistry Nobel Prize for super-resolved fluorescence imaging. As a newcomer, I was quite surprised that this nearly 400-year-old field could still catch the attention of Stockholm. After all, wasn’t a microscope just some lenses to

My history with GFP (and other members of the family)

Posted by Joachim Goedhart, on 7 July 2020

I first crossed paths with green fluorescent protein (GFP) while doing an internship in 1996 at the Max Planck Institute in Göttingen. I was chemically labeling epidermal growth factor (EGF) with the fluorescent dyes Cy3 and Cy5 to study growth-factor signaling. Two post-docs were discussing the use of GFP as an alternative fluorescent tag and

Fixation artifacts and how to minimize them

Posted by Heather Brown-Harding, on 7 July 2020

Sample preparation is the first step for having high-quality images that will impress everyone, but it is often overlooked. Many times I have tried to help others improve their microscope images, only to find out that improvement was not possible due to the quality of the sample. No matter how expensive your microscope is, you

Open microscopy across our journals

Posted by FocalPlane, on 3 July 2020

21-27 October marked International Open Access Week 2019. During this week many events were organised, stimulating discussion on open knowledge and connecting like-minded people and organisations that are all working towards greater accessibility of research. But of course important work in this area is being done all year round and we find ourselves in an

Career insight: bioimage analysis

Posted by Pradeep Rajasekhar, on 3 July 2020

I am a postdoctoral fellow based at the Monash Institute of Pharmaceutical Sciences (MIPS), Melbourne, Australia. I am originally from Kerala, India and travelled to Australia for my postgraduate studies almost 13 years ago. I came to Melbourne because of all the high quality and affordable educational opportunities, and fell in love with it for

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Microscopy-related articles from our journals

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples Development 2024 151: dev202307
filoVision – using deep learning and tip markers to automate filopodia analysis J Cell Sci 2024 137: jcs261274
C omputational tools for quantifying actin filament numbers, lengths, and bundling Biology Open 2024 13: bio.060267

Microscopy-related preprint highlights

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