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How I got into the Flamingo project, a new way of sharing custom-built light sheet microscopes

Posted by Michael Weber, on 12 October 2020

Sharing microscopy technology is crucial for biomedical research. This is the story how I joined Jan Huisken and his team to give other scientists quick access to custom-built light sheet microscopes.

LSFM series – Surfing on the data freak wave! Part II: Before imaging: Know your sample (geometry)

Posted by Elisabeth Kugler, on 10 October 2020

This post, part of the blog series "LSFM series – Surfing on the data freak wave!", discusses (a) Sample preparation, (b) Light interaction with matter, (c) sample alignment and (d) checking fluorescence and calibration

LSFM series – Surfing on the data freak wave! PART I: Knowing your turf, knowing your surf!

Posted by Elisabeth Kugler, on 5 September 2020

Here we present a series of five blog posts with tips and tricks about light sheet microscopy. 1. The basics of LSFM (Sept 2020) 2. Improving sample mounting (Oct 2020) 3. Calibration and Acquisition (Nov 2020) 4. Tailoring the data (Dec 2020) 5. What is next? AI and smarter than us LSFM (Jan 2021)

Lessons learned from an open-hardware project in microscopy: The mesoSPIM initiative

Posted by Fabian Voigt, on 1 July 2020

The mesoscale selective plane illumination microscope (mesoSPIM) is a highly versatile open-source light-sheet microscope for imaging large (cm-sized) cleared tissue samples. Using the documentation and software available via mesospim.org and on Github, interested labs can set up their own microscope. Currently, ten mesoSPIM instruments are in operation around the globe and several more are under

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Microscopy-related articles from our journals

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples Development 2024 151: dev202307
filoVision – using deep learning and tip markers to automate filopodia analysis J Cell Sci 2024 137: jcs261274
C omputational tools for quantifying actin filament numbers, lengths, and bundling Biology Open 2024 13: bio.060267

Microscopy-related preprint highlights

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