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Featured image with Thibault Dhellemmes and Jérémie Teillon

Posted by , on 1 March 2024

Our featured image shows the full morphology of Relaxin-3 neurons in an entire adult mouse brain. To observe the full depth of this brain, it was made transparent by applying an optical clearing technique called Adipoclear. The sample was observed on an Ultramicroscope II light sheet microscope and is a maximum intensity projection over the
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Postdoc position in imaging-based biochemistry and single molecule biophysics

Posted by , on 2 September 2022

Wellcome-funded postdoc position in the Centre for Mechanochemical Cell Biology at University of Warwick. Join us to use imaging-based approaches to understand how molecular motors organise microtubules. We are looking for a biochemist/biophysicist interested in single molecule imaging using TIRF and single molecule force measurements using optical trapping, or a cell biologist with expertise in
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Janelia+EMBL BioImaging Seminar Series: How We Started a Successful Seminar Series during the Pandemic

Posted by , on 25 January 2022

How we started a global BioImaging seminar series in the middle of a global pandemic...
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FocalPlane features... Pavel Tomancak

Posted by , on 7 June 2021

In case you missed ‘FocalPlane features…’ this month, here is the recording of the webinar given by Pavel Tomancak on Tuesday 1 June 2021. Light sheet microscopy has been over more than a decade producing stunning new views of the dynamics of living systems. I have been observing the rapid growth of this technology, contributed
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Part V The future: The hope of smart microscopes and phantoms

Posted by , on 24 April 2021

Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact: kugler.elisabeth@gmail.com; emmanuel.reynaud@ucd.ie We are reaching the end of our LSFM journey. Now it is time to look into the future, riding the plane of light! So, what is next for the LSFM? 1. Four-to-two, then four, then one. The story of SPIM, and LSFM per se,
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LSFM series – Part IV: Data post-processing: Cropping, tailoring, and trimming

Posted by , on 23 January 2021

Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact:kugler.elisabeth@gmail.com; emmanuel.reynaud@ucd.ie There is a very thin sheet of light between gathering data and hoarding. In Science, between pilling up manuscripts on desk, books on shelves and samples in cold freezer, most of us fit in the latter category. It is OK, if you have space available
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Postdoc in Bioimaging and 3D bioprinting

Posted by , on 20 January 2021

A 2-year postdoc position is available in the Microenvironmental Ecology and Symbiosis group(see https://twitter.com/microsensing) at the Department of Biology, University of Copenhagen Application deadline is February 1, earliest start can be April 1 or later in 2021.Apply via this link: https://employment.ku.dk/faculty/?show=153173 I am looking for talented scientists with a PhD (or in the final phase
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LSFM series – Part III: Image acquisition: Calibration and acquisition

Posted by , on 17 December 2020

Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact:kugler.elisabeth@gmail.com; emmanuel.reynaud@ucd.ie Now, we are getting closer to the point of setting up our sample in the chamber to image it. Now that we know which microscope we are about to use (Part I) and have mounted it the right way (Part II), we need to trust
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LSFM series – Surfing on the data freak wave! Part II: Before imaging: Know your sample (geometry)

Posted by , on 10 October 2020

This post, part of the blog series "LSFM series – Surfing on the data freak wave!", discusses (a) Sample preparation, (b) Light interaction with matter, (c) sample alignment and (d) checking fluorescence and calibration
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LSFM series – Surfing on the data freak wave! PART I: Knowing your turf, knowing your surf!

Posted by , on 5 September 2020

Here we present a series of five blog posts with tips and tricks about light sheet microscopy. 1. The basics of LSFM (Sept 2020) 2. Improving sample mounting (Oct 2020) 3. Calibration and Acquisition (Nov 2020) 4. Tailoring the data (Dec 2020) 5. What is next? AI and smarter than us LSFM (Jan 2021)
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