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LSFM series – Part IV: Data post-processing: Cropping, tailoring, and trimming

Posted by Elisabeth Kugler, on 23 January 2021

Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact:kugler.elisabeth@gmail.com; emmanuel.reynaud@ucd.ie There is a very thin sheet of light between gathering data and hoarding. In Science, between pilling up manuscripts on desk, books on shelves and samples in cold freezer, most of us fit in the latter category. It is OK, if you have space available

Postdoc in Bioimaging and 3D bioprinting

Posted by mkuhl, on 20 January 2021

A 2-year postdoc position is available in the Microenvironmental Ecology and Symbiosis group(see https://twitter.com/microsensing) at the Department of Biology, University of Copenhagen Application deadline is February 1, earliest start can be April 1 or later in 2021.Apply via this link: https://employment.ku.dk/faculty/?show=153173 I am looking for talented scientists with a PhD (or in the final phase

Technology highlights - Quantitative Phase Imaging (QPI)

Posted by Johanna Bischof, on 20 January 2021

Interview with Helena Chmelová, Ph.D. from the Light Microscopy Core facility at the Institute of Molecular Genetics of the Czech Academy of Sciences in Prague, Czech Republic. Can you tell us a little bit about yourself – where you work, what your research focus is? I work at the Light Microscopy Core Facility at the Institute of

The story behind ZeroCostDL4Mic, or How to get started with using Deep Learning for your microscopy data

Posted by Romain Laine, on 18 January 2021

We all hear again and again about Deep Learning (DL), how great it is, how it’s going to revolutionise science, engineering, medicine and work. But where and how is this all happening? If you wonder how you could get started on thinking, let alone using DL on your microscopy data, look no further, we have

LSFM series – Part III: Image acquisition: Calibration and acquisition

Posted by Elisabeth Kugler, on 17 December 2020

Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact:kugler.elisabeth@gmail.com; emmanuel.reynaud@ucd.ie Now, we are getting closer to the point of setting up our sample in the chamber to image it. Now that we know which microscope we are about to use (Part I) and have mounted it the right way (Part II), we need to trust

Technology highlights - Traction Force Microscopy (TFM)

Posted by Johanna Bischof, on 9 December 2020

Interview with Aki Stubb, Ph.D. Please tell us a bit about yourself and the facility where you work. My name is Aki Stubb and I am a post-doctoral fellow at the University of Cambridge, UK. I did my PhD in the group of Johanna Ivaska at the Turku Bioscience Centre, University of Turku and Åbo

Technology highlights - Fluorescence Lifetime Imaging (FLIM)

Posted by Johanna Bischof, on 25 November 2020

Interview with Prof. Dolf Weijers and Prof. Joris Sprakel from Wageningen University, The Netherlands Tell us a little bit about who you are and about the people involved in the work we are talking about today. This work is a collaboration between the groups of Prof. Dolf Weijers and Prof. Joris Sprakel at Wageningen University,

Technology highlights - Coherent Anti-Stokes Raman Spectroscopy (CARS)

Posted by Johanna Bischof, on 28 October 2020

Interview with Antti Isomäki, PhD from the Biomedicum Imaging Unit of the University of Helsinki, Finland and Dalibor Pánek, PhD from the BIOCEV in Prague, Czech Republic. Tell us a bit about who you are and where your facility is based. AI: My name is Antti Isomäki and I am an optical physicist by training.

LSFM series – Surfing on the data freak wave! Part II: Before imaging: Know your sample (geometry)

Posted by Elisabeth Kugler, on 10 October 2020

This post, part of the blog series "LSFM series – Surfing on the data freak wave!", discusses (a) Sample preparation, (b) Light interaction with matter, (c) sample alignment and (d) checking fluorescence and calibration

Technology highlights – Structured Illumination Microscopy (SIM)

Posted by Johanna Bischof, on 30 September 2020

Interview with Ivan Novotný, Ph.D., from the Light Microscopy Core facility at the Institute of Molecular Genetics, in Prague, Czech Republic. Please tell us a bit about yourself and the facility where you work. I am employed as an imaging specialist at the Light Microscopy Core facility at the Institute of Molecular Genetics of the

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Microscopy-related articles from our journals

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples Development 2024 151: dev202307
filoVision – using deep learning and tip markers to automate filopodia analysis J Cell Sci 2024 137: jcs261274
C omputational tools for quantifying actin filament numbers, lengths, and bundling Biology Open 2024 13: bio.060267

Microscopy-related preprint highlights

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Microscopy-related preprint highlights

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