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An interview with Alenka Lovy

Posted by , on 27 November 2022

MiniBio: Dr. Alenka Lovy is an Assistant Professor at the Center for Integrative Biology (CIB) and in charge of LiSIUM, LightSheet Imaging at Universidad Mayor. where together with a team of researchers, she is introducing this novel platform to the Latin American region. Alenka is originally from the Czech Republic. She did her early studies
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An interview with Ramón Ramírez

Posted by , on 8 November 2022

MiniBio: Dr. Ramon Ramirez is the Coordinator of the Microscopy Centre at Universidad Mayor in Santiago de Chile, and a leading member at LiSIUM (the light sheet imaging centre). He did his early career in Chile, at Pontificia Universidad Católica de Valparaíso where he studied a BSc in Biology. He later went to Madrid to
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Tissue clearing: what invisible samples reveal about biology

Posted by , on 8 September 2021

Written by Jorge Almagro and Hendrik Messal The tissues that constitute organs exist in our body in 3D. However, for practical reasons, histological analysis has been traditionally performed in 2D, slicing a few micrometer-thick sections to analyse them under the microscope. While this has been critical to understand our anatomy for centuries, it constitutes a simplification of
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FocalPlane features... Pavel Tomancak

Posted by , on 7 June 2021

In case you missed ‘FocalPlane features…’ this month, here is the recording of the webinar given by Pavel Tomancak on Tuesday 1 June 2021. Light sheet microscopy has been over more than a decade producing stunning new views of the dynamics of living systems. I have been observing the rapid growth of this technology, contributed
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Part V The future: The hope of smart microscopes and phantoms

Posted by , on 24 April 2021

Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact: kugler.elisabeth@gmail.com; emmanuel.reynaud@ucd.ie We are reaching the end of our LSFM journey. Now it is time to look into the future, riding the plane of light! So, what is next for the LSFM? 1. Four-to-two, then four, then one. The story of SPIM, and LSFM per se,
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LSFM series – Part IV: Data post-processing: Cropping, tailoring, and trimming

Posted by , on 23 January 2021

Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact:kugler.elisabeth@gmail.com; emmanuel.reynaud@ucd.ie There is a very thin sheet of light between gathering data and hoarding. In Science, between pilling up manuscripts on desk, books on shelves and samples in cold freezer, most of us fit in the latter category. It is OK, if you have space available
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LSFM series – Part III: Image acquisition: Calibration and acquisition

Posted by , on 17 December 2020

Elisabeth Kugler 1 and Emmanuel G. Reynaud 2 Contact:kugler.elisabeth@gmail.com; emmanuel.reynaud@ucd.ie Now, we are getting closer to the point of setting up our sample in the chamber to image it. Now that we know which microscope we are about to use (Part I) and have mounted it the right way (Part II), we need to trust
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How I got into the Flamingo project, a new way of sharing custom-built light sheet microscopes

Posted by , on 12 October 2020

Sharing microscopy technology is crucial for biomedical research. This is the story how I joined Jan Huisken and his team to give other scientists quick access to custom-built light sheet microscopes.
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LSFM series – Surfing on the data freak wave! Part II: Before imaging: Know your sample (geometry)

Posted by , on 10 October 2020

This post, part of the blog series "LSFM series – Surfing on the data freak wave!", discusses (a) Sample preparation, (b) Light interaction with matter, (c) sample alignment and (d) checking fluorescence and calibration
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LSFM series – Surfing on the data freak wave! PART I: Knowing your turf, knowing your surf!

Posted by , on 5 September 2020

Here we present a series of five blog posts with tips and tricks about light sheet microscopy. 1. The basics of LSFM (Sept 2020) 2. Improving sample mounting (Oct 2020) 3. Calibration and Acquisition (Nov 2020) 4. Tailoring the data (Dec 2020) 5. What is next? AI and smarter than us LSFM (Jan 2021)
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